ArticleeLife2019
A microfluidic device for inferring metabolic landscapes in yeast monolayer colonies.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Analysing and meta-analysing time-series data of microbial growth and gene expression from plate readers.PLoS computational biology · 2022Pooled it
- Dynamic patterns of gene expression match extracellular signals through push-pull regulation.PLoS genetics · 2025Article
- Article
- Substrate geometry affects population dynamics in a bacterial biofilm.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Optogenetic spatial patterning of cooperation in yeast populations.Nature communications · 2024Article
- CyberSco.Py an open-source software for event-based, conditional microscopy.Scientific reports · 2022Article
- Multiple nutrient transporters enable cells to mitigate a rate-affinity tradeoff.PLoS computational biology · 2022Article
- Preventing Photomorbidity in Long-Term Multi-color Fluorescence Imaging ofG3 (Bethesda, Md.) · 2020Article
- Observing Nutrient Gradients, Gene Expression and Growth Variation Using the "Yeast Machine" Microfluidic Device.Bio-protocol · 2020Article
- Aspects of Multicellularity inGenes · 2020Review
- Challenges and approaches in assessing the interplay between microorganisms and their physical micro-environments.Computational and structural biotechnology journal · 2020Review
- Chemotropism among populations of yeast cells with spatiotemporal resolution in a biofabricated microfluidic platform.Biomicrofluidics · 2020Article
- Diverse conditions support near-zero growth in yeast: Implications for the study of cell lifespan.Microbial cell (Graz, Austria) · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Microbial colonies are fascinating structures in which growth and internal organization reflect complex morphogenetic processes. Here, we generated a microfluidics device with arrays of long monolayer yeast colonies to further global understanding of how intercellular metabolic interactions affect the internal structure of colonies within defined boundary conditions. We observed the emergence of stable glucose gradients using fluorescently labeled hexose transporters and quantified the spatial correlations with intra-colony growth rates and expression of other genes regulated by glucose availability. These landscapes depended on the external glucose concentration as well as secondary gradients, for example amino acid availability. This work demonstrates the regulatory genetic networks governing cellular physiological adaptation are the key to internal structuration of cellular assemblies. This approach could be used in the future to decipher the interplay between long-range metabolic interactions, cellular development and morphogenesis in more complex systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.