ArticleBiomedical optics express2019
Second harmonic generation microscopy of early embryonic mouse hearts.
Article in Biomedical optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
4 citing papers in PubMed.
- Mouse embryo phenotyping with optical coherence tomography.Frontiers in cell and developmental biology · 2022Review
- Label-Free Multiphoton Microscopy: Much More Than Fancy Images.International journal of molecular sciences · 2021Review
- Label-free optical imaging in developmental biology [Invited].Biomedical optics express · 2020Review
- Recent progress in optical probing and manipulation of tissue: introduction.Biomedical optics express · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The understanding of biomechanical regulation of early heart development in genetic mouse models can contribute to improved management of congenital cardiovascular defects and embryonic cardiac failures in humans. The extracellular matrix (ECM), and particularly fibrillar collagen, are central to heart biomechanics, regulating tissue strength, elasticity and contractility. Here, we explore second harmonic generation (SHG) microscopy for visualization of establishing cardiac fibers such as collagen in mouse embryos through the earliest stages of development. We detected significant increase in SHG positive fibrillar content and organization over the first 24 hours after initiation of contractions. SHG microscopy revealed regions of higher fibrillar organization in regions of higher contractility and reduced fibrillar content and organization in mouse Mlc2a model with cardiac contractility defect, suggesting regulatory role of mechanical load in production and organization of structural fibers from the earliest stages. Simultaneous volumetric SHG and two-photon excitation microscopy of vital fluorescent reporter EGFP in the heart was demonstrated. In summary, these data set SHG microscopy as a valuable non-bias imaging tool to investigate mouse embryonic cardiogenesis and biomechanics.
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Registered trials
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