Evidence map›Paper›PMID 31258878›Full record

ArticleJournal of extracellular vesicles2019

High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer.

Aizea Morales-Kastresana, Thomas A Musich, Joshua A Welsh, William Telford, Thorsten Demberg, James C S Wood, Marty Bigos, Carley D Ross, Aliaksander Kachynski, Alan Dean and 26 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.

0numbers the graph read from it
0cells of the map it votes in
80citing papers in PubMed
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

80 citing papers in PubMed, 123 citations in OpenAlex.

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  15. Adenosine triphosphate-mediated signaling of P2X7 receptors controls donor extracellular vesicle release and major histocompatibility complex cross-decoration after allotransplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2025
    Article
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20 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

36 authors at 10 institutions in 1 country.

Aizea Morales-KastresanaVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.ORCID 0000-0003-1401-9805
Thomas A MusichVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.
Joshua A WelshVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.ORCID 0000-0002-1097-9756
William TelfordExperimental Immunology and Transplantation Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Thorsten DembergVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.
James C S WoodWake Forest School of Medicine Flow Cytometry Core, Winston Salem, NC, USA.
Marty BigosStanford University School of Medicine, Stanford, CA, USA.
Carley D RossBeckman Coulter, Fort Collins, CO, USA.
Aliaksander KachynskiBeckman Coulter, Fort Collins, CO, USA.
Alan DeanBeckman Coulter, Fort Collins, CO, USA.
Edward J FeltonBeth Israel Deaconess Medical Center, Boston, MA, USA.
Jonathan Van DykeUniversity of California, Davis, Sacramento, CA, USA.
John TiggesBeth Israel Deaconess Medical Center, Boston, MA, USA.
Vasilis ToxavidisBeth Israel Deaconess Medical Center, Boston, MA, USA.
David R ParksStanford University School of Medicine, Stanford, CA, USA.
W Roy OvertonQuantaCyte Corporation, NJ, USA.
Aparna H KesarwalaRadiation Oncology Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Gordon J FreemanDana-Farber Cancer Institute, Boston, MA, USA.
Ariel RosnerVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.
Stephen P PerfettoVaccine Research Center, National Institute of Allergy and Infectious Disease, NIH, Bethesda, MD, USA.
Lise PasquetVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.
Masaki TerabeVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.
Katherine McKinnonVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.
Veena KapoorExperimental Immunology and Transplantation Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Jane B TrepelDevelopmental Therapeutics Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Anu PuriBasic Research Lab, National Cancer Institute, NIH, Frederick, MD, USA.
Hisataka KobayashiMolecular Imaging Program, National Cancer Institute, NIH, Bethesda, MD, USA.
Bryant YungTheranostic Nanomedicine Section, National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD, USA.ORCID 0000-0003-2690-6778
Xiaoyuan ChenTheranostic Nanomedicine Section, National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD, USA.ORCID 0000-0002-9622-0870
Peter GuionRadiation Oncology Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Peter ChoykeMolecular Imaging Program, National Cancer Institute, NIH, Bethesda, MD, USA.
Susan J KnoxStanford University School of Medicine, Stanford, CA, USA.
Ionita GhiranBeth Israel Deaconess Medical Center, Boston, MA, USA.ORCID 0000-0001-6655-3298
Marjorie Robert-GuroffVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.
Jay A BerzofskyVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.
Jennifer C JonesVaccine Branch, National Cancer Institute, National Institutes of Health (NIH), Bethesda, MD, USA.ORCID 0000-0002-9488-7719
National Institutes of Health · USNational Cancer Institute · USBeth Israel Deaconess Medical Center · USBeckman Coulter Foundation · USStanford University · USNational Institute of Biomedical Imaging and Bioengineering · USDana-Farber Cancer Institute · USNational Institute of Allergy and Infectious Diseases · USUniversity of California, Davis · USWake Forest University · US

Funding

Prostate Cancer ImagingZIABC010655 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI CHOYKE, PETER L · 2009 to 2025
$49.2M
Laboratory of Molecular Imaging and NanomedicineZIAEB000073 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI CHEN, XIAOYUAN · 2010 to 2020
$32.8M
TIM recognition of phosphatidylserine regulates innate and adaptive immunityP01AI054456 · NIAID · STANFORD UNIVERSITY · PI CASASNOVAS, JOSE M · 2003 to 2014
$12.8M
Development and use of nanoFACS for analysis and sorting of nanoparticlesZIABC011502 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI JONES, JENNIFER · 2013 to 2025
$8.6M
Novel Combinations of Radiation and ImmunotherapyZIABC011503 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI JONES, JENNIFER · 2013 to 2025
$5.3M
Impact of circadian rhythm in obtaining reference profiles of exRNAs in healthy iU01HL126497 · NHLBI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI GHIRAN, IONITA CALIN · 2014 to 2018
$3.3M
TIM recognition of PtdSer on apoptotic cells and the regulation of immunityR01AI089955 · NIAID · DANA-FARBER CANCER INST · PI DEKRUYFF, ROSEMARIE H, FREEMAN, GORDON JAMES · 2010 to 2014
$2.1M
Four Laser, 18 Color Cell Sorter in the SSFFS10RR025518 · NCRR · STANFORD UNIVERSITY · PI HERZENBERG, LEONARD A · 2009 to 2009
$500k
EXTRAMURAL RESEARCH FACILITIES CONSTRUCTIONC06RR012088 · NCRR · UNIVERSITY OF CALIFORNIA DAVIS · PI CURRY, FITZ-ROY E · 1996 to 1996
–
Intramural NIH HHS ZIA BC011502Intramural NIH HHS ZIA BC011503NCRR NIH HHS C06 RR012088NCRR NIH HHS S10 RR025518NHLBI NIH HHS U01 HL126497NIAID NIH HHS P01 AI054456NIAID NIH HHS R01 AI089955
6 · The paper itself

Abstract

Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields of medicine as biomarkers and mediators of or treatments for disease. However, exosomes and small viruses fall below the detection limits of conventional flow cytometers due to the overlap of particle-associated scattered light signals with the detection of background instrument noise from diffusely scattered light. To identify, sort, and study distinct subsets of EVs and other nanoparticles, as individual particles, we developed nanoscale Fluorescence Analysis and Cytometric Sorting (nanoFACS) methods to maximise information and material that can be obtained with high speed, high resolution flow cytometers. This nanoFACS method requires analysis of the instrument background noise (herein defined as the "reference noise"). With these methods, we demonstrate detection of tumour cell-derived EVs with specific tumour antigens using both fluorescence and scattered light parameters. We further validated the performance of nanoFACS by sorting two distinct HIV strains to >95% purity and confirmed the viability (infectivity) and molecular specificity (specific cell tropism) of biological nanomaterials sorted with nanoFACS. This nanoFACS method provides a unique way to analyse and sort functional EV- and viral-subsets with preservation of vesicular structure, surface protein specificity and RNA cargo activity.

Indexed as

extracellular vesiclesflow cytometryNanofacsphenotypingsorting

Identifiers

PMID31258878
PMCPMC6586126
OpenAlexW2949462030

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.