Evidence map›Paper›PMID 31257504›Full record

ArticleMolecular medicine reports2019

miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase.

Chunli Xu, Chen Wang, Qiuyu Meng, Yuming Gu, Qiwei Wang, Wenjie Xu, Ying Han, Yong Qin, Jiao Li, Song Jia and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. AnFrontiers in genetics · 2024
    Article
  6. Evaluation of a Set of miRNAs in 26 Cases of Fatal Traumatic Brain Injuries.International journal of molecular sciences · 2023
    Article
  7. Review
  8. Review
  9. Neuronal microRNAs safeguard ER CaCellular and molecular life sciences : CMLS · 2022
    Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Chunli XuDepartment of Neurology, The Seventh People's Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Chen WangSchool of Life Science and Technology, School of Medicine, Tongji University, Shanghai 200092, P.R. China.
Qiuyu MengSchool of Life Science and Technology, School of Medicine, Tongji University, Shanghai 200092, P.R. China.
Yuming GuDepartment of Neurology, The Seventh People's Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Qiwei WangDepartment of Neurology, The Seventh People's Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Wenjie XuDepartment of Neurology, The Seventh People's Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Ying HanDepartment of Neurology, The Seventh People's Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Yong QinDepartment of Neurology, The Seventh People's Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Jiao LiTeaching Laboratory Center of Medicine and Life Science, School of Medicine, Tongji University, Shanghai 200092, P.R. China.
Song JiaTeaching Laboratory Center of Medicine and Life Science, School of Medicine, Tongji University, Shanghai 200092, P.R. China.
Jie XuTeaching Laboratory Center of Medicine and Life Science, School of Medicine, Tongji University, Shanghai 200092, P.R. China.
Yixin ZhouDepartment of Neurology, The Seventh People's Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs (miRNAs) have been found to play important regulatory roles in certain neurodegenerative diseases. The aim of the present study was to investigate the effect of miRNA‑153 (miR‑153) on the neural differentiation of HT‑22 cells. Overexpression of miR‑153 induced the differentiation of HT‑22 cells, increasing the number of protrusions and branches, reducing the S phase distribution of the cell cycle, and attenuating the cell proliferation rate as determined using the Cell Counting Kit‑8 assay. Furthermore, miR‑153 increased the expression of neuron‑specific γ‑enolase (NSE), neuronal nuclei (NeuN), and N‑ethylmaleimide‑sensitive fusion attachment protein 23 (SNAP23) and SNAP25 at the transcriptional and protein level by PCR and western blot analysis. Moreover, miR‑153 caused obvious upregulation of peroxiredoxin 5 (PRX5), which has been found to protect neural cells from death and apoptosis. miR‑153 promoted neural differentiation and protected neural cells by upregulating the neuron markers γ‑enolase, neuronal nuclei, and the functional proteins SNAP23, SNAP25 and PRX5. Therefore, miR‑153 may be a potential target for the treatment of certain neurodegenerative diseases.

Indexed as

NeurogenesisAnimalsCell LineCell ProliferationGene Expression Regulation, DevelopmentalHippocampusMiceMicroRNAsPhosphopyruvate HydrataseUp-RegulationMicroRNAsMIRN153 microRNA, mousePhosphopyruvate Hydratase

Identifiers

PMID31257504
PMCPMC6625396

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.