Evidence map›Paper›PMID 31257465›Full record

ArticleInternational journal of molecular medicine2019

Comparison of the therapeutic effects of adipose‑derived and bone marrow mesenchymal stem cells on erectile dysfunction in diabetic rats.

Sansan Chen, Jianbin Zhu, Mingzhu Wang, Yanting Huang, Zhuolin Qiu, Jingjing Li, Xinglu Chen, Huiying Chen, Mingyu Xu, Jun Liu and 5 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.

  1. Pooled it
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  7. Research Advances in Stem Cell Therapy for Erectile Dysfunction.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2024
    Review
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  9. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Sansan ChenDepartment of Urology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China.
Jianbin ZhuTechnology Center, Guangdong Vitalife Bio‑Tech Co., Ltd., Foshan, Guangdong 528200, P.R. China.
Mingzhu WangReproductive Center of Obstetrics and Gynecology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Yanting HuangClinical Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China.
Zhuolin QiuReproductive Center of Obstetrics and Gynecology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Jingjing LiTechnology Center, Guangdong Vitalife Bio‑Tech Co., Ltd., Foshan, Guangdong 528200, P.R. China.
Xinglu ChenClinical Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China.
Huiying ChenInstitute of Biotherapy, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Mingyu XuInstitute of Biotherapy, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Jun LiuDermatology Hospital of Southern Medical University, Guangzhou, Guangdong 510091, P.R. China.
Miaoqin SheGuangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510660, P.R. China.
Hongwei LiInstitute of Biotherapy, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Xiaorong YangClinical Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China.
Yi WangCentral Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China.
Xiangsheng CaiInstitute of Biotherapy, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Southern Medical University · CNFirst Affiliated Hospital of Guangdong Pharmaceutical University · CNChinese Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the present study was to compare the effects of adipose‑derived mesenchymal stem cell (ADSC) and bone marrow mesenchymal stem cell (BMSC) transplantation into the corpora cavernosa of diabetic rats with erectile function. ADSCs and BMSCs were isolated and identified by flow cytometry. Rats with streptozocin‑induced diabetes were screened using apomorphine to obtain a rat model of diabetic erectile dysfunction, followed by transplantation of ADSCs and BMSCs into the corpora cavernosa. Two weeks later, the rats were again injected with apomorphine, the intracavernous pressure (ICP) and mean arterial pressure (MAP) of the penile tissue were measured, and the corpus cavernosum tissues were harvested. Angiogenic endothelial nitric oxide synthase (eNOS) expression was detected by western blotting and immunofluorescence analysis. The blood vessels in the corpus cavernosum were observed following hematoxylin and eosin (H&E) staining, and the expression of collagen was detected by Sirius Red staining. The cellular ultrastructure was examined by transmission electron microscopy. Intracavernous injection of ADSCs significantly increased ICP and ICP/MAP. Western blotting and immunofluorescence results revealed that ADSC treatment improved the expression of eNOS in the penile tissue of diabetic rats. The H&E staining results demonstrated that ADSC treatment promoted revascularization of the corpus cavernosum, and the results of Sirius Red staining revealed that ADSC treatment reduced penile collagen in diabetic rats. Transmission electron microscopy examination revealed that the ultrastructure of the tissues in the ADSC‑treated group was more complete compared with that in the untreated diabetic model group. In conclusion, ADSCs were found to be more effective compared with BMSCs in treating diabetes‑related erectile dysfunction.

Indexed as

Diabetes ComplicationsMesenchymal Stem CellsMesenchymal Stem Cell TransplantationAdipose TissueAnimalsBiomarkersCollagenErectile DysfunctionMaleNitric Oxide Synthase Type IIIPenile ErectionRatsBiomarkersCollagenNitric Oxide Synthase Type III

Identifiers

PMID31257465
PMCPMC6658012
OpenAlexW2953348599

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.