ArticleJournal of experimental botany2019
Targeted manipulation of bZIP53 DNA-binding properties influences Arabidopsis metabolism and growth.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Integrated Gene Regulatory Network Analysis Reveals Coordinated Transcriptional Reprogramming in thePlants (Basel, Switzerland) · 2026Article
- Regulatory Mechanisms of Free Umami Amino Acid Accumulation in Fresh Waxy Kernels: Insights from Transcriptome and Metabolomics Analyses.Foods (Basel, Switzerland) · 2025Article
- Primary metabolism determines the outcome of salicylic acid-mediated immune induction.bioRxiv : the preprint server for biology · 2025Article
- Single-cell transcriptional profiling in Arabidopsis root exposed to boron toxicity at seedling stages.Plant molecular biology · 2025Article
- Regulation of HvASN1 expression by bZIP transcription factors during barley embryo development and germination.Planta · 2025Article
- Optimizing data integration improves gene regulatory network inference in Arabidopsis thaliana.Bioinformatics (Oxford, England) · 2024Article
- Functional Identification ofPlants (Basel, Switzerland) · 2024Article
- Identification of bZIP Transcription Factors That Regulate the Development of Leaf Epidermal Cells inInternational journal of molecular sciences · 2024Article
- Integrated Metabolome and Transcriptome Analyses Reveal Amino Acid Biosynthesis Mechanisms during the Physiological Maturity of Grains in Yunnan Hulled Wheat (International journal of molecular sciences · 2023Article
- A pear S1-bZIP transcription factor PpbZIP44 modulates carbohydrate metabolism, amino acid, and flavonoid accumulation in fruits.Horticulture research · 2023Article
- Overexpression of theBiology · 2023Article
- Transcriptomic and metabolomic analyses reveal the mechanism of uniconazole inducing hypocotyl dwarfing by suppressing BrbZIP39-Frontiers in plant science · 2022Article
- S1-bZIP Transcription Factors Play Important Roles in the Regulation of Fruit Quality and Stress Response.Frontiers in plant science · 2021Review
- Genome-Wide Characterization and Expression Analysis ofFrontiers in genetics · 2021Article
- bZIP edgetic mutations: at the frontier of plant metabolism, development and stress trade-off.Journal of experimental botany · 2019Article
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7 authors.
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Abstract
bZIP transcription factors regulate diverse processes in eukaryotic cells. Arabidopsis bZIP members of the C and S1 groups form heterodimers and synergistically control metabolic reprogramming during stress responses. However, their functional characterization is complicated due to an overlapping heterodimerization network and high redundancy. In this study, we develop a simple but powerful approach for generating dominant negative mutants of bZIP factors with high specificity. By applying in vitro DNA-binding, reporter gene and protoplast two-hybrid assays, and plant mutant analysis, we show that phosphorylation-mimicking substitution of conserved serines in the DNA-binding domain of bZIP monomeric subunits suffices for the disruption of the interaction of both bZIP homo- and heterodimers with cognate DNA. This results in the transcriptional inactivation of target genes. The dominant-negative effect is achieved by the unaltered function of the intrinsic nuclear localization signal and dimerization properties of the mutated bZIP protein. Our findings not only reveal an additional regulatory mechanism of bZIP10 intracellular localization, but also provide evidence of the involvement of bZIP53 in the diurnal adjustments of amino acid metabolism. Our data demonstrate the advantages and the suitability of this new approach for the artificial inactivation of bZIP transcription factors in plants, and it may also be of use for other organisms.
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