ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2019
Comparison of standard and on-plate extraction protocols for identification of mastitis-causing bacteria by MALDI-TOF MS.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Incidence of Streptococcus spp. and whole genome sequencing of Streptococcus agalactiae isolated from cow's milk samples in Brazil.Veterinary research communications · 2026Article
- Association Between Antimicrobial Susceptibility Test Results and Bacteriological Cure of Mild and Moderate Bovine Clinical Mastitis.Veterinary sciences · 2026Article
- Draft genomes of two Streptococcus agalactiae from bovine mastitis in the North-West Province, South Africa.BMC genomic data · 2025Article
- Influence of Sub-Inhibitory Concentrations of Sanitizers and Oxacillin on the Resistance of Methicillin-ResistantVeterinary sciences · 2025Article
- Injection site abscesses associated with commensal and environmental bacteria following intramuscular vaccination in horses.Veterinary and animal science · 2025Article
- Subclinical Mastitis Related toVeterinary sciences · 2025Article
- Machine Learning Techniques Associated With Infrared Thermography to Optimize the Diagnosis of Bovine Subclinical Mastitis.Veterinary medicine international · 2025Article
- Economic impact of staphylococcal and mammaliicoccal subclinical mastitis in dairy herds from Northeast Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Outbreak of Esophagitis and Ingluvitis Caused byVeterinary sciences · 2024Article
- Apparent prevalence and selected risk factors of methicillin-resistantJDS communications · 2023Article
- Genetic and Phenotypic Characterization of Subclinical Mastitis-Causing Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- The Emergence of Antimicrobial Resistance and Virulence Characteristics inAntibiotics (Basel, Switzerland) · 2023Article
- Molecular characterization of persistent subclinical mastitis-causing Staphylococcus aureus from dairy farms.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Association between Mastitis Occurrence in Dairy Cows and Bedding Characteristics of Compost-Bedded Pack Barns.Pathogens (Basel, Switzerland) · 2023Article
- The Impact of Selective Dry Cow Therapy Adopted in a Brazilian Farm on Bacterial Diversity and the Abundance of Quarter Milk.Veterinary sciences · 2022Article
- Antimicrobial resistance profile of extended-spectrum beta-lactamases, adenosine-monophosphate-cyclic, and carbapenemase-producing Gram-negative bacteria isolated from domestic animals.Veterinary world · 2021Article
- MALDI-TOF MS identification ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Evaluation of Chromogenic Culture Media for Rapid Identification of Gram-Positive Bacteria Causing Mastitis.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The objective was to compare standard versus on-plate sample preparation protocols for identification of mastitis bacteria by MALDI-TOF MS. A total of 186 bacterial isolates from cows with subclinical mastitis were identified by MALDI-TOF MS after preparation using two extraction protocols. On-plate protocol was performed by applying the bacterial colony directly from the culture plate onto the plate spot. For the standard protocol, lysis of bacterial colonies using reagents was performed in a cryotube, and the resulting extract was applied onto the plate spot for analysis. The on-plate protocol showed a similar bacteria identification rate (91.4%, n = 170/186) in comparison to the standard (94.6%, n = 176/186). Identification was higher for both protocols when scores used for species-level identification (≥ 2.0) was reduced to genus-level (≥ 1.7); genus-level identification score rate increased from 94.6 to 100% when using the standard protocol, and from 91.4 to 94.6% when using the on-plate protocol. However, when compared standard (as gold standard) versus on-plate protocol, genus-level identification score rate ranged from 87.1 to 89.8%. Therefore, when the on-plate protocol fails to identify any specie, the standard extraction may be more suitable as a reference protocol for use. Strategy for increasing identification with the on-plate protocol may include upgrading the reference database library. Choice of protocol for preparation may be influenced by the bacterial type to be identified. Standard and on-plate extraction protocols of bacterial ribosomal proteins associated with MALDI-TOF MS might be alternatives to conventional microbiology methods for identification of subclinical mastitis pathogens.
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