ArticleMolecular biotechnology2019
Identification of T-DNA Insertion Site and Flanking Sequence of a Genetically Modified Maize Event IE09S034 Using Next-Generation Sequencing Technology.
Article in Molecular biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton.Plants (Basel, Switzerland) · 2026Article
- Unintended effects of exogenous DNA integration in transgenic papaya analyzed at DNA and transcriptional levels.BMC plant biology · 2026Article
- Unbiased Long-Read Whole-Genome Sequencing Enables High-Resolution Mapping of Transgene Concatenation and Off-target Genomic Disruption in a Mouse Model.Computational and structural biotechnology journal · 2026Article
- Efficient transgenic maize (Zea Mays L.) detection using low-depth next-generation sequencing.Scientific reports · 2025Article
- Deciphering the complex molecular architecture of the genetically modified soybean FG72 through paired-end whole genome sequencing.Food chemistry. Molecular sciences · 2025Article
- Transgene Mapping in Animals: What to Choose?International journal of molecular sciences · 2025Review
- Analysis of the Candidate Genes and Underlying Molecular Mechanism of P198, an RNAi-Related Dwarf and Sterile Line.International journal of molecular sciences · 2023Article
- Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize.International journal of molecular sciences · 2023Review
- Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences.Foods (Basel, Switzerland) · 2023Review
- Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing.Frontiers in plant science · 2023Article
- Efficient identification of genomic insertions and flanking regions through whole-genome sequencing in three transgenic soybean events.Transgenic research · 2021Article
- Defining the 'HoneySweet' insertion event utilizing NextGen sequencing and a de novo genome assembly of plum (Prunus domestica).Horticulture research · 2021Article
- Elucidation of genomic organizations of transgenic soybean plants through de novo genome assembly with short paired-end reads.Molecular breeding : new strategies in plant improvement · 2021Article
- Using Combined Methods of Genetic Mapping and Nanopore-Based Sequencing Technology to Analyze the Insertion Positions ofFrontiers in plant science · 2021Article
- Strategies to produce T-DNA free CRISPRed fruit trees via Agrobacterium tumefaciens stable gene transfer.Scientific reports · 2020Article
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5 authors.
Funding
Abstract
Molecular characteristics including information of insertion site, flanking sequence, and copy numbers are the base for the safety assessment and subsequent monitoring of genetically modified organisms (GMOs), which has to be revealed thoroughly in a case-by-case manner. Although both polymerase chain reaction (PCR)-based and next-generation sequencing (NGS)-based approaches are proven to be effective in the molecular characterization of most of GM events, they often fail to work with GM maize events, mainly due to the genome complexity. In this study, by using NGS, we successfully identified the 3' end T-DNA insertion site and flanking sequence of a GM maize event IE09S034, which were confirmed by PCR amplification and Sanger sequencing. Notably, insertions of unintended exogenous elements were revealed in this event although the single copy of target exogenous genes was also confirmed by digital PCR. The output of this study provides novel and important genetic evidence for the safety assessment and monitoring of GM maize event IE09S034.
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