ArticleProceedings of the National Academy of Sciences of the United States of America1988
Partial reversion of the transformed phenotype in HRAS-transfected tumorigenic cells by transfer of a human gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 59 citations in OpenAlex.
- High expression of PLA2G16 is associated with a better prognosis in HER2-positive breast cancer.Journal of thoracic disease · 2017Article
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- Growth-inhibitory activity and downregulation of the class II tumor-suppressor gene H-rev107 in tumor cell lines and experimental tumors.The Journal of cell biology · 1997Article
- Suppression of anchorage-independent growth after gene transfection.British journal of cancer · 1993Article
- Suppressible and nonsuppressible autocrine mast cell tumors are distinguished by insertion of an endogenous retroviral element (IAP) into the interleukin 3 gene.The Journal of experimental medicine · 1993Article
- Fte-1, a v-fos transformation effector gene, encodes the mammalian homologue of a yeast gene involved in protein import into mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Short-term treatment with gamma interferon induces stable reversion of ras-transformed mouse fibroblasts.Journal of virology · 1991Article
- Molecular and functional analysis of tumor-suppressor genes by transfection.Environmental health perspectives · 1991Review
- Functional in vitro assays for the isolation of cell transformation effector and suppressor genes.Environmental health perspectives · 1991Review
- Prohibitin, an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells.Molecular and cellular biology · 1991Article
- The current state of oncogenes and cancer: experimental approaches for analyzing oncogenetic events in human cancer.Cancer metastasis reviews · 1990Review
- Tumor suppression involves down-regulation of interleukin 3 expression in hybrids between autocrine mastocytoma and interleukin 3-dependent parental mast cells.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Role of a tumor-suppressor gene in the negative control of anchorage-independent growth of Syrian hamster cells.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Detection of genes with a potential for suppressing the transformed phenotype associated with activated ras genes.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Recessive mechanisms of malignancy.British journal of cancer · 1988Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transformed phenotype of rat FE-8 cells transfected by an activated human HRAS gene was suppressed upon fusion with normal cells. An experimental approach was developed to identify and isolate a human gene capable of suppressing the transforming activity of the HRAS oncogene in FE-8 cells. Genomic DNA from human placenta was introduced into FE-8 cells by cotransfection with the plasmid pY3 conferring hygromycin B resistance. Transfectants were selected in medium containing hygromycin B. HRAS-transformed FE-8 cells showed an increased sensitivity toward ouabain when compared to their normal counterparts. Therefore, the population of transfected hygromycin B-resistant cells was treated with ouabain to eliminate cells with a transformed phenotype. Ouabain selection resulted in a small number of cell clones exhibiting a more normal phenotype. The clones had lost the morphology of transformed cells and required anchorage for growth. The tumorigenicity of transfectants in nude mice was reduced but not completely abolished. FE-8 revertants continued to express the p21 RAS protein. Human repetitive sequences contained in the DNA of a secondary transfectant were used for isolation of the suppressor gene from reverted FE-8 cells. The cloned DNA fragment was transfected into tumorigenic FE-8 cells and conferred a partial reversion of the transformed phenotype.
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