ArticleRedox biology2019
ReactELISA method for quantifying methylglyoxal levels in plasma and cell cultures.
Article in Redox biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Recent Advance in the Sample Pretreatments for Drug Analysis in Zebrafish.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Scavenging methylglyoxal improves bone quality and defect healing in diabetic mice.Bone reports · 2025Article
- Identification of Methylglyoxal Reactive Proteins with Photocaged Glycating Agents.Chembiochem : a European journal of chemical biology · 2025Article
- Statins could improve short-term prognosis in patients with traumatic brain injury: a propensity-matched cohort study.Inflammopharmacology · 2025Article
- Novel Technique for Simultaneous Ethylene Glycol and Its Metabolites Determination in Human Whole Blood and Urine Samples Using GC-QqQ-MS/MS.Journal of xenobiotics · 2024Article
- Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons.bioRxiv : the preprint server for biology · 2023Article
- Article
- A Ratiometric Fluorescence Probe for Selective Detection of ex vivo Methylglyoxal in Diabetic Mice.ChemistryOpen · 2022Article
- Chemoselective and Highly Sensitive Quantification of Gut Microbiome and Human Metabolites.Angewandte Chemie (International ed. in English) · 2021Article
- Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS.Molecules (Basel, Switzerland) · 2021Review
- Sirtuin 2 Regulates Protein LactoylLys Modifications.Chembiochem : a European journal of chemical biology · 2021Article
- Non-enzymatic Lysine Lactoylation of Glycolytic Enzymes.Cell chemical biology · 2020Article
- Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methylglyoxal (MG) is a toxic glycolytic by-product associated with increased levels of inflammation and oxidative stress and has been linked to ageing-related diseases, such as diabetes and Alzheimer's disease. As MG is a highly reactive dicarbonyl compound, forming both reversible and irreversible adducts with a range of endogenous nucleophiles, measuring endogenous levels of MG are quite troublesome. Furthermore, as MG is a small metabolite it is not very immunogenic, excluding conventional ELISA for detection purposes, thus only more instrumentally demanding LC-MS/MS-based methods have demonstrated convincing quantitative data. In the present work we develop a novel bifunctional MG capture probe as well as a high specificity monoclonal antibody to finally setup a robust reaction-based ELISA (ReactELISA) method for detecting the highly reactive and low-level (nM) metabolite MG in human biological specimens. The assay is tested and validated against the current golden standard LC-MS/MS method in human blood plasma and cell-culture media. Furthermore, we demonstrate the assays ability to measure small perturbations of MG levels in growth media caused by a small molecule drug buthionine sulfoximine (BSO) of current clinical relevance. Finally, the assay is converted into a homogenous (no-wash) AlphaLISA version (ReactAlphaLISA), which offers the potential for operationally simple screening of further small molecules capable of perturbing cellular MG. Such compounds could be of relevance as probes to gain insight into MG metabolism as well as drug-leads to alleviate ageing-related diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.