ArticleJournal of assisted reproduction and genetics2019
Human OVGP1 enhances tyrosine phosphorylation of proteins in the fibrous sheath involving AKAP3 and increases sperm-zona binding.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- A special perspective on human fertilization: The role of sperm capacitation proteins and channels: A narrative review.International journal of reproductive biomedicine · 2025Review
- Precursor A-Kinase Anchor Protein 4 as a Predictive Biomarker of Post-Thaw Semen Quality in Goats.Veterinary sciences · 2025Article
- Function of manchette and intra-manchette transport in spermatogenesis and male fertility.Cell communication and signaling : CCS · 2025Review
- Evaluation of novel biomarkers for early pregnancy outcome prediction†.Biology of reproduction · 2024Article
- Extracellular vesicles affecting embryo developmentFrontiers in pharmacology · 2024Review
- The sperm-interacting proteome in the bovine isthmus and ampulla during the periovulatory period.Journal of animal science and biotechnology · 2023Article
- Recombinant human OVGP1 increases intracellular calcium and further potentiates the effects of progesterone on human sperm.Journal of assisted reproduction and genetics · 2022Article
- IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice.Developmental biology · 2022Article
- LRRC46 Accumulates at the Midpiece of Sperm Flagella and Is Essential for Spermiogenesis and Male Fertility in Mouse.International journal of molecular sciences · 2022Article
- Spatiotemporal profiling of the bovine oviduct fluid proteome around the time of ovulation.Scientific reports · 2022Article
- The role of oviduct-specific glycoprotein (OVGP1) in modulating biological functions of gametes and embryos.Histochemistry and cell biology · 2022Review
- Ouabain-induced activation of phospholipase C zeta and its contributions to bovine sperm capacitation.Cell and tissue research · 2021Article
- Article
- Identification of Photoperiod-Induced LncRNAs and mRNAs in Pituitary Pars Tuberalis of Sheep.Frontiers in veterinary science · 2021Article
- Altered Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia.Computational and mathematical methods in medicine · 2021Article
- Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site.International journal of molecular sciences · 2020Article
- Superficial and profound considerations about sperm quality.Journal of assisted reproduction and genetics · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo investigate if the recombinant human oviduct-specific glycoprotein (rHuOVGP1)-enhanced tyrosine-phosphorylated (pY) proteins are components of specific structure(s) of the sperm tail and if rHuOVGP1 binds to the oocyte and enhances sperm-egg binding.
methodsImmunofluorescent staining and confocal microscopy were performed to examine the localization of pY proteins, outer dense fiber (ODF), and A-Kinase Associated Protein 3 (AKAP3) in human sperm during capacitation. Western blot and immunoprecipitation were employed to analyze protein levels of pY proteins and AKAP3. Immunofluorescent staining was performed to examine the binding of rHuOVGP1 to human oocytes. The effect of rHuOVGP1 on enhancing sperm-zona binding was examined using hemizona assay.
resultspY proteins were detected mainly in the fibrous sheath (FS) surrounding the ODF with a relatively weak immunoreaction in the neck and mid-piece. Western blot analysis revealed co-migration of the pY 105 kDa protein with AKAP3, which was further confirmed by immunoprecipitation correlating immunofluorescent results of co-localization of pY proteins with AKAP3 in the sperm tail. rHuOVGP1 binds specifically to the zona pellucida (ZP) of human oocytes. Prior incubation of sperm and/or ZP with rHuOVGP1 increased sperm-egg binding.
conclusionsThe present study revealed that one of the major rHuOVGP1-enhanced pY proteins could be AKAP3 of the FS and that rHuOVGP1 is capable of binding to human ZP and its presence in the medium results in an increase in sperm-zona binding. Supplement of rHuOVGP1 in in vitro fertilization media could be beneficial for enhancement of the fertilizing ability of human sperm.
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