ArticleMicrobial cell factories2019
Increased production of ganoderic acids by overexpression of homologous farnesyl diphosphate synthase and kinetic modeling of ganoderic acid production in Ganoderma lucidum.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 37 citations in OpenAlex.
- Enhanced Production and Profiling of Ganoderic Acids inJournal of fungi (Basel, Switzerland) · 2026Article
- Overexpression of the ATP-Citrate Lyase Gene Enhances Ganoderic Acid Biosynthesis in Ganoderma lingzhi.Molecular biotechnology · 2026Article
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- Comprehensive genomic identification and functional analysis ofChinese herbal medicines · 2026Article
- Blue Light Receptor WC-2 Regulates Ganoderic Acid Biosynthesis inJournal of fungi (Basel, Switzerland) · 2025Article
- CRISPR/Cas9-mediated genome editing in Ganoderma lucidum: recent advances and biotechnological opportunities.World journal of microbiology & biotechnology · 2025Review
- Towards engineering agaricomycete fungi for terpenoid production.Journal of industrial microbiology & biotechnology · 2024Review
- Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects.Journal of fungi (Basel, Switzerland) · 2024Review
- Increasing the production of the bioactive compounds in medicinal mushrooms: an omics perspective.Microbial cell factories · 2023Review
- A Review ofBiomolecules · 2022Review
- Transcriptome Analyses Revealed the Key Metabolic Genes and Transcription Factors Involved in Terpenoid Biosynthesis in Sacred Lotus.Molecules (Basel, Switzerland) · 2022Article
- Evaluation of Conditions to Improve Biomass Production by Submerged Culture ofMicroorganisms · 2022Article
- Comparative transcriptome analysis of genes and metabolic pathways involved in sporulation in Ganoderma lingzhi.G3 (Bethesda, Md.) · 2022Article
- A methyltransferase LaeA regulates ganoderic acid biosynthesis inFrontiers in microbiology · 2022Article
- Biosynthesis and regulation of terpenoids from basidiomycetes: exploration of new research.AMB Express · 2021Review
- Targeted Gene Insertion and Replacement in the Basidiomycete Ganoderma lucidum by Inactivation of Nonhomologous End Joining Using CRISPR/Cas9.Applied and environmental microbiology · 2021Article
- Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum.Microbial cell factories · 2021Article
- Identification of an Oxidosqualene Cyclase Gene Involved in Steroidal Triterpenoid Biosynthesis inGenes · 2021Article
- Article
- Transcriptome dynamics and metabolite analysis revealed the candidate genes and regulatory mechanism of ganoderic acid biosynthesis during liquid superficial-static culture of Ganoderma lucidum.Microbial biotechnology · 2021Article
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4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundGanoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpression on GA production was investigated by developing the corresponding mathematical models.
resultsThe maximum levels of total GAs and individual GAs (GA-T, GA-S, and GA-Me) in the transgenic strain were 2.76 mg/100 mg dry weight (DW), 41 ± 2, 21 ± 5, and 28 ± 1 μg/100 mg DW, respectively, which were increased by 2.28-, 2.27-, 2.62-, and 2.80-folds compared with those in the control. Transcription levels of squalene synthase (SQS) and lanosterol synthase (LS) genes during GA biosynthesis were upregulated by 2.28- and 1.73-folds, respectively, in the transgenic G. lucidum. In addition, the developed unstructured models had a satisfactory fit for the process of GA production in submerged cultures of G. lucidum. Analysis of the kinetic process showed that FPS gene overexpression had a stronger positive impact on GA production compared with its influence on cell growth. Also, FPS gene overexpression led to a higher non-growth-associated-constant β (1.151) over the growth-associated-constant α (0.026) in the developed models.
conclusionsFPS gene overexpression is an effective strategy to improve the production of GAs in G. lucidum. The developed mathematical models are useful for developing a better GA production process in future large-scale bioreactors.
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