ArticleBMC microbiology2019
Assessment of the impact of different fecal storage protocols on the microbiota diversity and composition: a pilot study.
Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis.Microbiology spectrum · 2023Pooled it
- Azithromycin Exposure Induces Transient Microbial Composition Shifts and Decreases the Airway Microbiota Resilience from Outdoor PMMicrobiology spectrum · 2023Trial
- The cervicovaginal gut microbiota axis as a key determinant of systemic physiology.Gut microbes · 2026Review
- Multidisciplinary Delphi consensus statement on minimal standards for clinical metadata and end points in microbiome studies.Nature reviews. Gastroenterology & hepatology · 2026Review
- Exploring gut microbiota profile induced by antipsychotics in schizophrenic patients: insights from an Eastern European pilot study.BMC psychiatry · 2025Article
- Toward standardized methods in canine vaginal microbiome research: evaluation of storage, host DNA depletion, and database selection.Microbiology spectrum · 2025Article
- Stability of oral and fecal microbiome at room temperature: impact on diversity.Frontiers in microbiomes · 2025Article
- Exploring gut microbiota alterations in Parkinson's disease: insights from a 16S amplicon sequencing Eastern European pilot study.Frontiers in neuroscience · 2025Article
- Article
- Dissecting the human gut microbiome to better decipher drug liability: A once-forgotten organ takes center stage.Journal of advanced research · 2023Review
- Comparison of DNA extraction methods for 16S rRNA gene sequencing in the analysis of the human gut microbiome.Scientific reports · 2023Article
- Best practice for wildlife gut microbiome research: A comprehensive review of methodology for 16S rRNA gene investigations.Frontiers in microbiology · 2023Review
- Reduced abundance ofFrontiers in microbiomes · 2023Article
- Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies.Journal of animal science · 2022Review
- Storage of soil microbiome for application in sustainable agriculture: prospects and challenges.Environmental science and pollution research international · 2022Review
- Standards for Collection, Preservation, and Transportation of Fecal Samples in TCM Clinical Trials.Frontiers in cellular and infection microbiology · 2022Article
- The maintenance of microbial community in human fecal samples by a cost effective preservation buffer.Scientific reports · 2021Article
- Critical evaluation of faecal microbiome preservation using metagenomic analysis.ISME communications · 2021Article
- The landscape of microbiota research in Iran; a bibliometric and network analysis.Journal of diabetes and metabolic disorders · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFecal samples are currently the most commonly studied proxy for gut microbiota. The gold standard of sample handling and storage for microbiota analysis is maintaining the cold chain during sample transfer and immediate storage at - 80 °C. Gut microbiota studies in large-scale, population-based cohorts require a feasible sample collection protocol. We compared the effect of three different storage methods and mock shipment: immediate freezing at - 80 °C, in 95% ethanol stored at room temperature (RT) for 48 h, and on blood collection card stored at RT for 48 h, on the measured composition of fecal microbiota of eight healthy, female volunteers by sequencing the V4 region of the 16S rRNA gene on an Illumina MiSeq.
resultsShared operational taxonomic units (OTUs) between different methods were 68 and 3% for OTUs > 0.01 and < 0.01% mean relative abundance within each group, respectively. α and β-diversity measures were not significantly impacted by different storage methods. With the exception of Actinobacteria, fecal microbiota profiles at the phylum level were not significantly affected by the storage method. Actinobacteria was significantly higher in samples collected on card compared to immediate freezing (1.6 ± 1.1% vs. 0.4 ± 0.2%, p = 0.005) mainly driven by expansion of Actinobacteria relative abundance in fecal samples stored on card in two individuals. There was no statistically significant difference at lower taxonomic levels tested.
conclusionConsistent results of the microbiota composition and structure for different storage methods were observed. Fecal collection on card could be a suitable alternative to immediate freezing for fecal microbiota analysis using 16S rRNA gene amplicon sequencing.
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