Evidence map›Paper›PMID 31251980›Full record

ArticleNeurotoxicology and teratology

Anesthetics disrupt growth cone guidance cue sensing through actions on the GABA

Jing Xu, Michael Xu, YuChia Wang, R Paige Mathena, Jieqiong Wen, Pengbo Zhang, Orion Furmanski, C David Mintz

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurotoxicology and teratology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Jing XuDepartment of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, 710004, China; Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Michael XuDepartment of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
YuChia WangDepartment of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
R Paige MathenaDepartment of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Jieqiong WenDepartment of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, 710004, China; Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Pengbo ZhangDepartment of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, 710004, China.
Orion FurmanskiDepartment of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
C David MintzDepartment of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: cmintz2@jhmi.edu.
Johns Hopkins University · USJohns Hopkins Medicine · USSecond Affiliated Hospital of Xi'an Jiaotong University · CN

Funding

Sleep and Circadian Dysfunction, Brain and Neurobehavioral Development in AutismP50HD103538 · NICHD · HUGO W. MOSER RES INST KENNEDY KRIEGER · PI Stewart H Mostofsky · 2020 to 2026
$9.9M
Program in Postdoctoral Research Training in AnesthesiologyT32GM075774 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI SAPNA R KUDCHADKAR, Emmett E Whitaker · 2006 to 2026
$8.1M
Mechanisms of Developmental Anesthesia ToxicityR01GM120519 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI MINTZ, CYRUS DAVID · 2016 to 2020
$2.1M
The effects of anesthetics on brain developmentK08GM104329 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI MINTZ, CYRUS DAVID · 2013 to 2016
$626k
NICHD NIH HHS P50 HD103538NIGMS NIH HHS K08 GM104329NIGMS NIH HHS R01 GM120519NIGMS NIH HHS T32 GM075774
6 · The paper itself

Abstract

backgroundGeneral anesthetics (GAs) may exert harmful effects on the developing brain by disrupting neuronal circuit formation. Anesthetics that act on γ-aminobutyric acid (GABA) receptors can interfere with axonal growth cone guidance, a critical process in the assembly of neuronal circuitry. Here we investigate the mechanism by which isoflurane prevents sensing of the repulsive guidance cue, Semaphorin 3A (Sema3A).

methodsGrowth cone sensing was assayed by measuring growth cone collapse in dissociated neocortical cultures exposed to recombinant Sema3A in the presence or absence of isoflurane and/or a panel of reagents with specific actions on components of the GABA receptor and chloride ion systems.

resultsIsoflurane exposure prevents Sema3A induced growth cone collapse. A GABA

conclusionOur data suggest that the effects of isoflurane on growth cone sensing are mediated by the α2 subunit of the GABA

Indexed as

Anesthetics, InhalationAnimalsAxon GuidanceChloridesGrowth ConesIsofluranePrimary Cell CultureRats, Sprague-DawleyReceptors, GABA-ASemaphorin-3AAnesthetics, InhalationChloridesIsofluraneReceptors, GABA-ASema3a protein, ratSemaphorin-3AAnesthesiaChloride gradientGABA(A)RsGrowth coneNeurotoxicity

Identifiers

PMID31251980
PMCPMC6650745
OpenAlexW2955157868

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.