ArticleJournal of the American Society for Mass Spectrometry2019
Data Processing Pipeline for Lipid Profiling of Carotid Atherosclerotic Plaque with Mass Spectrometry Imaging.
Article in Journal of the American Society for Mass Spectrometry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Spatial localization of arachidonic acid in human carotid atherosclerotic plaques reveals a pro-inflammatory metabolic program in macrophages.Frontiers in molecular biosciences · 2026Article
- ADAMTS- 1 rs402007 Polymorphism Modulates Carotid Plaque Vulnerability and Atorvastatin Efficacy in Cerebral Infarction Patients.Translational stroke research · 2025Article
- Spatial omics strategies for investigating human carotid atherosclerotic disease.Clinical and translational medicine · 2025Review
- Oleic acid triggers metabolic rewiring of T cells poising them for T helper 9 differentiation.iScience · 2024Article
- Recent Analytical Methodologies in Lipid Analysis.International journal of molecular sciences · 2024Review
- Spatial lipidomics of coronary atherosclerotic plaque development in a familial hypercholesterolemia swine model.Journal of lipid research · 2024Article
- Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.Scientific reports · 2023Article
- Pathophysiology of Atherosclerosis.International journal of molecular sciences · 2022Review
- MALDI MSI for a fresh view on atherosclerotic plaque lipids.Pflugers Archiv : European journal of physiology · 2022Article
- Comparative lipid profiling of murine and human atherosclerotic plaques using high-resolution MALDI MSI.Pflugers Archiv : European journal of physiology · 2022Article
- Loss of OTUD6B Stimulates Angiogenesis and Promotes Diabetic Atherosclerosis.Diabetes, metabolic syndrome and obesity : targets and therapy · 2022Article
- De Novo Sphingolipid Biosynthesis in Atherosclerosis.Advances in experimental medicine and biology · 2022Article
- Effects of fatty acids on T cell function: role in atherosclerosis.Nature reviews. Cardiology · 2021Review
- Micro Spectroscopic Photoacoustic (μsPA) imaging of advanced carotid atherosclerosis.Photoacoustics · 2021Article
- Mass Spectrometry Imaging as a Tool to Investigate Region Specific Lipid Alterations in Symptomatic Human Carotid Atherosclerotic Plaques.Metabolites · 2021Article
- Atheroma-Specific Lipids inJournal of the American Society for Mass Spectrometry · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Atherosclerosis is a lipid and inflammation-driven disease of the arteries that is characterized by gradual buildup of plaques in the vascular wall. A so-called vulnerable plaque, consisting of a lipid-rich necrotic core contained by a thin fibrous cap, may rupture and trigger thrombus formation, which can lead to ischemia in the heart (heart attack) or in the brain (stroke). In this study, we present a protocol to investigate the lipid composition of advanced human carotid plaques using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI), providing a framework that should enable the discrimination of vulnerable from stable plaques based on lipid composition. We optimized the tissue preparation and imaging methods by systematically analyzing data from three specimens: two human carotid endarterectomy samples (advanced plaque) and one autopsy sample (early stage plaque). We show a robust data reduction method and evaluate the variability of the endarterectomy samples. We found diacylglycerols to be more abundant in a thrombotic area compared to other plaque areas and could distinguish advanced plaque from early stage plaque based on cholesteryl ester composition. We plan to use this systematic approach to analyze a larger dataset of carotid atherosclerotic plaques.
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