Evidence map›Paper›PMID 31250305›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting.

Francheska Son, Katharine Umphred-Wilson, Jae-Hyuck Shim, Stanley Adoro

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Biogenesis of Extracellular Vesicles.Sub-cellular biochemistry · 2021
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Francheska SonDepartment of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
Katharine Umphred-WilsonDepartment of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
Jae-Hyuck ShimDivision of Rheumatology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Stanley AdoroDepartment of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA. sxa726@case.edu.
Case Western Reserve University · USUniversity of Massachusetts Chan Medical School · US

Funding

GRADUATE TRAINING IN CELLULAR AND MOLECULAR BIOLOGYT32GM008056 · NIGMS · CASE WESTERN RESERVE UNIVERSITY · PI DIEHL, JOHN ALAN, SNIDER, MARTIN D · 1985 to 2019
$7.1M
Mechanisms of tunable posttranslational control of T-cell homeostasis and toleranceR01AI143992 · NIAID · CASE WESTERN RESERVE UNIVERSITY · PI XIAO, TSAN SAM · 2019 to 2023
$2.2M
Probing the role of the IRE1alpha-XBP1 pathway in normal and malignant hematopoiesisK22CA218467 · NCI · CASE WESTERN RESERVE UNIVERSITY · PI ADORO, STANLEY · 2017 to 2018
$385k
NCI NIH HHS K22 CA218467NIAID NIH HHS R01 AI143992NIGMS NIH HHS T32 GM008056
6 · The paper itself

Abstract

The charged multivesicular body protein-5 (CHMP5) is a member of the endosomal-sorting complex required for transport (ESCRT) that controls membrane-scission events in eukaryotic cells. Recent studies have revealed novel functions of CHMP5 beyond its role in the ESCRT machinery, notably as a critical nonenzymatic regulator of the ubiquitination and subsequent degradation of proteins in immune cells. Here we describe an immunoprecipitation and western blot methodology for assessing CHMP5 activity on client protein ubiquitination in T lymphocytes.

Indexed as

UbiquitinationBlotting, WesternEndosomal Sorting Complexes Required for TransportEndosomesHEK293 CellsHumansImmunoprecipitationJurkat CellsT-LymphocytesCHMP5 protein, humanEndosomal Sorting Complexes Required for TransportCHMP5DeubiquitinasePosttranslational modificationT lymphocytesUbiquitination

Identifiers

PMID31250305
PMCPMC10216709
OpenAlexW2953453988

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.