Evidence map›Paper›PMID 31250299›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.

Shai Adar-Levor, Inna Goliand, Michael Elbaum, Natalie Elia

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Shai Adar-LevorDepartment of Life Sciences and NIBN, Ben Gurion University of the Negev, Beer Sheva, Israel.
Inna GoliandDepartment of Life Sciences and NIBN, Ben Gurion University of the Negev, Beer Sheva, Israel.
Michael ElbaumDepartment of Materials and Interfaces, Weizmann Institute of Science, Rehovot, Israel.
Natalie EliaDepartment of Life Sciences and NIBN, Ben Gurion University of the Negev, Beer Sheva, Israel. elianat@post.bgu.ac.il.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ESCRT machinery mediates scission of the intercellular bridge that connects two daughter cells at the end of cytokinesis. Structured illumination microscopy (SIM) and cryo-soft-X-ray tomography (cryo-SXT) have been used in recent years to study the topology of ESCRT-driven cytokinetic abscission. These studies revealed that the intercellular bridge is occupied by cortical rings and spiral-like filaments and that ESCRTs form ring-like structures in this region during abscission. In this chapter, we provide two protocols: a protocol for determining the spatial organization of specific ESCRT components at the intercellular bridge using SIM and a protocol for resolving the ultrastructural organization of cortical filaments at the intercellular bridge using cryo-SXT.

Indexed as

CytokinesisAnimalsCryoelectron MicroscopyDogsEndosomal Sorting Complexes Required for TransportGenes, ReporterGreen Fluorescent ProteinsHeLa CellsHumansIntravital MicroscopyLuminescent AgentsMadin Darby Canine Kidney CellsOptical ImagingTomography, X-RayEndosomal Sorting Complexes Required for TransportGreen Fluorescent ProteinsLuminescent AgentsCell divisionCorrelative imagingCryoCytokinesisESCRTESCRT-IIIFluorescence imagingImmunostainingMembrane fissionSoft-X-raySuper-resolution microscopySynchronizationTomography

Identifiers

PMID31250299

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.