Evidence map›Paper›PMID 31250293›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative Light Electron Microscopy.

Céline Largeau, Emmanuel Culetto, Renaud Legouis

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors at 1 institution in 2 countries.

Céline LargeauDiabetes and Islet Biology Group, Faculty of Medicine and Health, NHMRC Clinical Trials Centre, The University of Sydney, Camperdown, NSW, Australia.
Emmanuel CulettoInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ.Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France.
Renaud LegouisInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ.Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France. renaud.legouis@i2bc.paris-saclay.fr.
Institut de Biologie Intégrative de la Cellule · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this chapter, we report a protocol to perform correlative light electron microscopy (CLEM) on adult Caenorhabditis elegans. We use a specific fixation protocol, which preserves both the GFP fluorescence and the structural integrity of the samples. Thin sections are first analyzed by light microscopy to detect GFP-tagged proteins and, subsequently, with transmission electron microscopy (TEM) to characterize the ultrastructural anatomy of cells. The superimposition of light and electron images allows determining the subcellular localization of the fluorescent protein.We used CLEM to characterize the subcellular localization of the C. elegans ESCRT-II component VPS-36. VPS-36 protein localization in C. elegans muscle cell is strongly correlated with the sarcoplasmic reticulum network. Together with genetic evidences, the CLEM data support a role for ESCRT-II proteins in sarcoplasmic reticulum membrane shaping.

Indexed as

AnimalsCaenorhabditis elegansCryopreservationEndosomal Sorting Complexes Required for TransportGreen Fluorescent ProteinsImage Processing, Computer-AssistedMicroscopy, Electron, TransmissionMicroscopy, FluorescenceMolecular ImagingSarcoplasmic ReticulumTissue EmbeddingEndosomal Sorting Complexes Required for TransportGreen Fluorescent ProteinsElectron microscopyESCRT-IIFreeze substitutionGMA resinGreen fluorescent proteinHigh pressure freezingMuscleSarcoplasmic reticulumVPS-36

Identifiers

PMID31250293
OpenAlexW2954167812

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.