ArticleExtremophiles : life under extreme conditions2019
Reference genes for real-time RT-PCR expression studies in an Antarctic Pseudomonas exposed to different temperature conditions.
Article in Extremophiles : life under extreme conditions, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 19 citations in OpenAlex.
- From genome to lifestyle: adaptive strategies ofApplied and environmental microbiology · 2026Article
- Quantitative Evaluation of Endogenous Reference Genes for RT-qPCR and ddPCR Gene Expression Under Polyextreme Conditions Using Anaerobic Halophilic AlkalithermophileMicroorganisms · 2025Article
- Identification and validation of reference genes for qPCR of Pseudomonas aeruginosa L10 under varying n-hexadecane concentrations.BMC microbiology · 2025Article
- Kraft lignin biobleaching by a dye-decolorizing peroxidase from the Antarctic Pseudomonas sp. AU10 strain.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Temperature-dependent iron motion in extremophile rubredoxins - no need for 'corresponding states'.Scientific reports · 2024Article
- Phenotypic and resistome analysis of antibiotic and heavy metal resistance in the Antarctic bacterium Pseudomonas sp. AU10.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482.Scientific reports · 2021Article
- Comprehensive evaluation of candidate reference genes for quantitative real-time PCR-based analysis in Caucasian clover.Scientific reports · 2021Article
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3 authors at 1 institution in 1 country.
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Abstract
Psychrophilic and psychrotolerant bacteria from permanently cold environments may be the most abundant extremophiles on Earth and yet little is known on how they cope with temperature stress. Real-time reverse transcription PCR (RT-qPCR) is a powerful technique that could shed light on this matter but it requires pre-validated reference genes for normalization of data to get accurate results. In this study, we assessed the expression stability of eight candidate genes for the psychrotolerant Antarctic isolate Pseudomonas sp. AU10 during exponential growth under 4 °C and 30 °C, and after a cold-shock. Using the software programs BestKeeper and geNorm we validated recA, ftsZ, 16S rRNA, and rpoD as reference genes and we suggested the combination of recA and ftsZ for qPCR data normalization. Our results provide a starting point for gene expression studies in Antarctic Pseudomonas concerning temperature-related physiology and also for the validation of reference genes in other cold-adapted bacterial species.
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