ArticleBiotechnology for biofuels2019
Metatranscriptomics of the Hu sheep rumen microbiome reveals novel cellulases.
Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 80 citations in OpenAlex.
- The developmental trajectory and maturation of the Hulunbuir sheep (Ovis aries) microbiome.Animal microbiome · 2026Article
- Metagenomic and metabolomic analyses of rumen fiber digestion in Mongolian cattle fed fresh grass versus hay.Microbiology spectrum · 2026Article
- Precise Delivery of Nitric Oxide Controlled by Bioorthogonal Endocellulase Ameliorates Hindlimb Ischemia.Bioengineering (Basel, Switzerland) · 2026Article
- Metatranscriptomics reveals the horse gut RNA virome and a viral sharing network with human and domestic animals.Frontiers in veterinary science · 2026Article
- Functional dynamics reveal the response of the crabapple (mSystems · 2025Article
- Exploration of Multi-Source Lignocellulose-Degrading Microbial Resources and Bioaugmentation Strategies: Implications for Rumen Efficiency.Animals : an open access journal from MDPI · 2025Review
- Ruminal digestion, gastrointestinal microbial profile, and metabolic pathways after the introduction of silicon-containing ultrafine particles into bull.Veterinary world · 2025Article
- Chinese herbal medicine promotes growth by improving nutrient utilization and rumen microbiota in suckling lambs.Frontiers in microbiology · 2025Article
- Metatranscriptomics-guided discovery and characterization of a polyphenol-metabolizing gut microbial enzyme.Cell host & microbe · 2024Article
- Expression and characterization of a novel microbial GH9 glucanase, IDSGLUC9-4, isolated from sheep rumen.Animal bioscience · 2024Article
- Application of Pan-Omics Technologies in Research on Important Economic Traits for Ruminants.International journal of molecular sciences · 2024Review
- Bovine reproductive tract and microbiome dynamics: current knowledge, challenges, and its potential to enhance fertility in dairy cows.Frontiers in microbiomes · 2024Review
- High concentrate diets altered the structure and function of rumen microbiome in goats.Frontiers in microbiology · 2024Article
- Enhancement effect of AgO nanoparticles on fermentative cellulase activity from thermophilic Bacillus subtilis Ag-PQ.Journal, genetic engineering & biotechnology · 2023Article
- Microbiome Diversity and Cellulose Decomposition Processes by Microorganisms on the Ancient Wooden Seawall of Qiantang River of Hangzhou, China.Microbial ecology · 2023Article
- Characterization of Two Novel Rumen-Derived Exo-Polygalacturonases: Catalysis and Molecular Simulations.Microorganisms · 2023Article
- Genetic-Phenotype Analysis ofFoods (Basel, Switzerland) · 2023Article
- Production, purification, characterization and application of two novel endoglucanases from buffalo rumen metagenome.Journal of animal science and biotechnology · 2023Article
- Comparative analysis of rumen metagenome, metatranscriptome, fermentation and methane yield in cattle and buffaloes fed on the same diet.Frontiers in microbiology · 2023Article
- Heterologous expression and characterization of two novel glucanases derived from sheep rumen microbiota.World journal of microbiology & biotechnology · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCellulosic biomass has great potential as a renewable biofuel resource. Robust, high-performance enzymes are needed to effectively utilize this valuable resource. In this study, metatranscriptomics was used to explore the carbohydrate-active enzymes (CAZymes), especially glycoside hydrolases (GHs), present in the rumen microbiome of Hu sheep. Select CAZymes were experimentally verified and characterized after cloning and expression in
resultsThe metatranscriptomes of six Hu sheep rumen microbiomes yielded 42.3 Gbp of quality-checked sequence data that represented in total 2,380,783 unigenes after de novo assembling using Trinity and clustered with CD-HIT-EST. Annotation using the CAZy database revealed that 2.65% of the unigenes encoded GHs, which were assigned to 111 different CAZymes families.
conclusionThe metatranscriptomic results revealed that the rumen microbiome of Hu sheep encoded a repertoire of new enzymes capable of cellulose degradation and metatranscriptomics was an effective method to discover novel cellulases for biotechnological applications.
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