ArticleNature methods2019
Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells.
Article in Nature methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed.
- Gradient-driven microfluidic framework for the screening of ratiometric drug combinations towards precision oncology in paediatric leukaemia.Nature biomedical engineering · 2026Article
- Optogenetic BMP4 models symmetry breaking and primitive streak formation in hPSCs.bioRxiv : the preprint server for biology · 2026Article
- Development of a deep neural network model for simultaneous analysis of extracellular analyte gradients for a population of cells.Artificial intelligence in the life sciences · 2026Article
- A transgene-free, human peri-gastrulation embryo model presents trilaminar embryonic disc-, amnion- and yolk sac-like structures.Nature cell biology · 2026Article
- Strategies to control cellular spatial organization in microphysiological systems.Microsystems & nanoengineering · 2026Review
- From Preservation to Creation: The Expanding Frontier of Fertility Preservation - Proceedings of the 2Facts, views & vision in ObGyn · 2026Article
- Progress in stem cell-based embryo models and their applications in developmental biology and biomedicine.Nature reviews. Molecular cell biology · 2026Review
- Optogenetic WNT signaling drives germ layer self-organization in a human gastruloid model.bioRxiv : the preprint server for biology · 2026Article
- Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model.Science advances · 2025Article
- Bioengineering gradients for controlled embryo and organ modeling.Current opinion in biomedical engineering · 2025Article
- Organoid-based tissue engineering for advanced tissue repair and reconstruction.Materials today. Bio · 2025Review
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- The building blocks of embryo models: embryonic and extraembryonic stem cells.Cell discovery · 2025Review
- A microfluidic platform for anterior-posterior human endoderm patterning via countervailing morphogen gradientsiScience · 2025Article
- Synergistic potential of stem cells and microfluidics in regenerative medicine.Molecular and cellular biochemistry · 2025Review
- Organ-on-a-Chip Applications in Microfluidic Platforms.Micromachines · 2025Review
- Article
- Non-Invasive Quality Control of Organoid Cultures Using Mesofluidic CSTR Bioreactors and High-Content Imaging.Advanced materials technologies · 2025Article
- Microgels With Electrostatically Controlled Molecular Affinity to Direct Morphogenesis.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Poly(norepinephrine)-Mediated Universal Surface Modification for Patterning Human Pluripotent Stem Cell Culture and Differentiation.ACS biomaterials science & engineering · 2024Article
34 more citing papers are in PubMed but not listed here.
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hPSC) colonies to spatiotemporally controlled morphogen gradients generated from artificial signaling centers. In response to a localized source of bone morphogenetic protein 4 (BMP4), hPSC colonies reproducibly break their intrinsic radial symmetry to produce distinct, axially arranged differentiation domains. Counteracting sources of the BMP antagonist NOGGIN enhance this spatial control of cell fate patterning. We also show how morphogen concentration and cell density affect the BMP response and germ layer patterning. These results demonstrate that the intrinsic capacity of stem cells for self-organization can be extrinsically controlled through the use of engineered signaling centers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.