Evidence map›Paper›PMID 31248989›Full record

ArticleThe Journal of biological chemistry2019

Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues.

Matthew Blahut, Courtney E Wise, Michael R Bruno, Guangchao Dong, Thomas M Makris, Patrick A Frantom, Jack A Dunkle, F Wayne Outten

Open access · hybridAbstract read
In one paragraph

Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 40 citations in OpenAlex.

  1. Article
  2. Inactivation of ornithine aminotransferase by (1Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2026
    Article
  3. SmsB-SmsC machinery inApplied and environmental microbiology · 2025
    Article
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  16. Fe-S Protein Synthesis in Green Algae Mitochondria.Plants (Basel, Switzerland) · 2021
    Review
  17. Methods to Investigate the Kinetic Profile of Cysteine Desulfurases.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  18. Fe-S cluster biogenesis by the bacterial Suf pathway.Biochimica et biophysica acta. Molecular cell research · 2020
    Review
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Matthew BlahutDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208.
Courtney E WiseDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208.
Michael R BrunoDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, Alabama 35487.
Guangchao DongDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208.
Thomas M MakrisDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208.
Patrick A FrantomDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, Alabama 35487.
Jack A DunkleDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, Alabama 35487. Electronic address: jadunkle@ua.edu.
F Wayne OuttenDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208. Electronic address: woutten@sc.edu.
University of South Carolina · USUniversity of Alabama · US

Funding

Conformational dynamics and allosteric regulation during stress-responsive metallocofactor assemblyR01GM112919 · NIGMS · UNIVERSITY OF ALABAMA IN TUSCALOOSA · PI Patrick Frantom · 2015 to 2026
$2.8M
Phase II SER-CAT Optimization: Acquisition of a next generation area detectorS10RR028976 · NCRR · UNIVERSITY OF GEORGIA · PI WANG, BI-CHENG · 2010 to 2010
$1.5M
Acquition of a microdiffractometer for SER-CATS10RR025528 · NCRR · UNIVERSITY OF GEORGIA · PI ROSE, JOHN PATRICK · 2009 to 2009
$414k
NCRR NIH HHS S10 RR025528NCRR NIH HHS S10 RR028976NIGMS NIH HHS R01 GM112919
6 · The paper itself

Abstract

Iron-sulfur (Fe-S) clusters are necessary for the proper functioning of numerous metalloproteins. Fe-S cluster (Isc) and sulfur utilization factor (Suf) pathways are the key biosynthetic routes responsible for generating these Fe-S cluster prosthetic groups in

Indexed as

Models, MolecularAmino Acid SubstitutionCatalysisCatalytic DomainCrystallography, X-RayEscherichia coliLyasesMutation, MissenseLyasesselenocysteine lyasecysteine desulfurasecysteine sulfur bond cleavageenzyme catalysisenzyme mechanismiron-sulfur proteinPLPPLP-dependent sulfur abstractionpre-steady-state kineticsSufSX-ray crystallography

Identifiers

PMID31248989
PMCPMC6699847
OpenAlexW2955153270

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.