ArticlePLoS neglected tropical diseases2019
Elucidating diversity in the class composition of the minicircle hypervariable region of Trypanosoma cruzi: New perspectives on typing and kDNA inheritance.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 19 citations in OpenAlex.
- Deciphering Trypanosoma lainsoni kDNA minicircles: insights into genetic diversity, mRNA editing, and molecular diagnosis.Parasite (Paris, France) · 2026Article
- Deep Sequencing of mHVRs for Trypanosoma cruzi Genotyping.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Genetic Diversity ofLife (Basel, Switzerland) · 2024Review
- High-throughput analysis of the Trypanosoma cruzi minicirculome (mcDNA) unveils structural variation and functional diversity.Scientific reports · 2024Article
- New insights intoFrontiers in immunology · 2024Review
- Wide reference databases for typing Trypanosoma cruzi based on amplicon sequencing of the minicircle hypervariable region.PLoS neglected tropical diseases · 2023Article
- Deep kinetoplast genome analyses result in a novel molecular assay for detectingNAR genomics and bioinformatics · 2022Article
- Reproduction in Trypanosomatids: Past and Present.Biology · 2021Review
- Serological Approaches for Trypanosoma cruzi Strain Typing.Trends in parasitology · 2021Review
- Guide RNA Repertoires in the Main Lineages ofFrontiers in cellular and infection microbiology · 2021Article
- The Complete Mitochondrial DNA ofFrontiers in cellular and infection microbiology · 2021Article
- Ecological divergence and hybridization of NeotropicalProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I.PLoS neglected tropical diseases · 2020Article
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6 authors at 2 institutions in 1 country.
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Abstract
backgroundTrypanosoma cruzi, the protozoan causative of Chagas disease, is classified into six main Discrete Typing Units (DTUs): TcI-TcVI. This parasite has around 105 copies of the minicircle hypervariable region (mHVR) in their kinetoplastic DNA (kDNA). The genetic diversity of the mHVR is virtually unknown. However, cross-hybridization assays using mHVRs showed hybridization only between isolates belonging to the same genetic group. Nowadays there is no methodologic approach with a good sensibility, specificity and reproducibility for direct typing on biological samples. Due to its high copy number and apparently high diversity, mHVR becomes a good target for typing. METHODOLOGY/PRINCIPAL
findingsAround 22 million reads, obtained by amplicon sequencing of the mHVR, were analyzed for nine strains belonging to six T. cruzi DTUs. The number and diversity of mHVR clusters was variable among DTUs and even within a DTU. However, strains of the same DTU shared more mHVR clusters than strains of different DTUs and clustered together. In addition, hybrid DTUs (TcV and TcVI) shared similar percentages (1.9-3.4%) of mHVR clusters with their parentals (TcII and TcIII). Conversely, just 0.2% of clusters were shared between TcII and TcIII suggesting biparental inheritance of the kDNA in hybrids. Sequencing at low depth (20,000-40,000 reads) also revealed 95% of the mHVR clusters for each of the analyzed strains. Finally, the method revealed good correlation in cluster identity and abundance between different replications of the experiment (r = 0.999). CONCLUSIONS/SIGNIFICANCE: Our work sheds light on the sequence diversity of mHVRs at intra and inter-DTU level. The mHVR amplicon sequencing workflow described here is a reproducible technique, that allows multiplexed analysis of hundreds of strains and results promissory for direct typing on biological samples in a future. In addition, such approach may help to gain knowledge on the mechanisms of the minicircle evolution and phylogenetic relationships among strains.
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