ArticlePloS one2019
Pore-scale hydrodynamics influence the spatial evolution of bacterial biofilms in a microfluidic porous network.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Dimensional analysis as a tool for biofilm comparative analysis: bridging the interdisciplinary divides.Journal of bacteriology · 2026Review
- Soil as a Battlefield and a Reservoir: Linking Soil Components to the Epidemiology of Soilborne Plant Diseases.Microbial ecology · 2026Review
- Investigating the effects of soil microstructures on bacterial growth via microfluidic channels and an agent-based model.Scientific reports · 2025Article
- Microenvironments on individual sand grains enhance nitrogen loss in coastal sediments.Scientific reports · 2025Article
- Hierarchical Structure of the Program Used by Filamentous Fungi to Navigate in Confining Microenvironments.Biomimetics (Basel, Switzerland) · 2025Article
- New Cyclam-Based Fe(III) Complexes Coatings TargetingMolecules (Basel, Switzerland) · 2025Article
- Microfluidics for studying the deep underground biosphere: from applications to fundamentals.FEMS microbiology ecology · 2024Review
- Fungi and bacteria occupy distinct spatial niches within carious dentin.PLoS pathogens · 2024Article
- The mechanism of biofilm detachment in porous medium under flow field.Biomicrofluidics · 2024Article
- Microfluidic approaches in microbial ecology.Lab on a chip · 2024Review
- Emerging sensing, imaging, and computational technologies to scale nano-to macroscale rhizosphere dynamics - Review and research perspectives.Soil biology & biochemistry · 2024Article
- Studying root-environment interactions in structured microdevices.Journal of experimental botany · 2023Article
- Experimental and numerical investigation of polymer pore-clogging in micromodels.Scientific reports · 2023Article
- Ultraviolet control of bacterial biofilms in microfluidic chips.Biomicrofluidics · 2023Article
- Assessment of the Antibiofilm Performance of Chitosan-Based Surfaces in Marine Environments.International journal of molecular sciences · 2022Article
- Competition between growth and shear stress drives intermittency in preferential flow paths in porous medium biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Synthetic Soil Aggregates: Bioprinted Habitats for High-Throughput Microbial Metaphenomics.Microorganisms · 2022Article
- Microfluidic devices for studying bacterial taxis, drug testing and biofilm formation.Microbial biotechnology · 2022Review
- Habitat geometry in artificial microstructure affects bacterial and fungal growth, interactions, and substrate degradation.Communications biology · 2021Article
- Microfluidic chips provide visual access to in situ soil ecology.Communications biology · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria occupy heterogeneous environments, attaching and growing within pores in materials, living hosts, and matrices like soil. Systems that permit high-resolution visualization of dynamic bacterial processes within the physical confines of a realistic and tractable porous media environment are rare. Here we use microfluidics to replicate the grain shape and packing density of natural sands in a 2D platform to study the flow-induced spatial evolution of bacterial biofilms underground. We discover that initial bacterial dispersal and grain attachment is influenced by bacterial transport across pore space velocity gradients, a phenomenon otherwise known as rheotaxis. We find that gravity-driven flow conditions activate different bacterial cell-clustering phenotypes depending on the strain's ability to product extracellular polymeric substances (EPS). A wildtype, biofilm-producing bacteria formed compact, multicellular patches while an EPS-defective mutant displayed a linked-cell phenotype in the presence of flow. These phenotypes subsequently influenced the overall spatial distribution of cells across the porous media network as colonies grew and altered the fluid dynamics of their microenvironment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.