ArticlePlant direct2017
High-throughput droplet microfluidics screening platform for selecting fast-growing and high lipid-producing microalgae from a mutant library.
Article in Plant direct, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 83 citations in OpenAlex.
- A Scalable Droplet Microfluidic Platform That Overcomes Large-Cell Constraints for Long-Term Plant Cell Culture and Embryogenesis Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Polychip-A High-Throughput Droplet Microfluidics Platform for Interrogating Microbial Interactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Microfluidic droplet cultivation preserves microalgae diversity in screening systems.Scientific reports · 2025Article
- Size-independent and automated single-colony-resolution microdroplet dispensing.Lab on a chip · 2025Article
- Rapid filamentous fungi gene knockout identification through high-throughput droplet microfluidics.Analytica chimica acta · 2025Article
- Elevated accumulation of lutein and zeaxanthin in a novel high-biomass yielding strain Dunaliella sp. ZP-1 obtained through EMS mutagenesis.Biotechnology for biofuels and bioproducts · 2025Article
- Microfluidic droplets with amended culture media cultivate a greater diversity of soil microorganisms.Applied and environmental microbiology · 2025Article
- High-Efficiency Interdigitated Electrode-Based Droplet Merger for Enabling Error-Free Droplet Microfluidic Systems.Analytical chemistry · 2024Article
- Droplet Microfluidics for High-Throughput Screening and Directed Evolution of Biomolecules.Micromachines · 2024Review
- Multiple Rounds ofACS synthetic biology · 2023Article
- Tutorial on Lateral Dielectrophoretic Manipulations in Microfluidic Systems.IEEE transactions on biomedical circuits and systems · 2023Article
- Monodisperse Micro-Droplet Generation in Microfluidic Channel with Asymmetric Cross-Sectional Shape.Micromachines · 2023Article
- Droplet Detection and Sorting System in Microfluidics: A Review.Micromachines · 2022Review
- Article
- Strain Development in Microalgal Biotechnology-Random Mutagenesis Techniques.Life (Basel, Switzerland) · 2022Review
- High-Aspect-Ratio Microfluidic Channel with Parallelogram Cross-Section for Monodisperse Droplet Generation.Biosensors · 2022Article
- High-throughput selection of cells based on accumulated growth and division using PicoShell particles.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Droplet Microfluidics for Microbial Biotechnology.Advances in biochemical engineering/biotechnology · 2022Article
- Droplet digital microfluidic system for screening filamentous fungi based on enzymatic activity.Microsystems & nanoengineering · 2022Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofuels derived from microalgal lipids have demonstrated a promising potential as future renewable bioenergy. However, the production costs for microalgae-based biofuels are not economically competitive, and one strategy to overcome this limitation is to develop better-performing microalgal strains that have faster growth and higher lipid content through genetic screening and metabolic engineering. In this work, we present a high-throughput droplet microfluidics-based screening platform capable of analyzing growth and lipid content in populations derived from single cells of a randomly mutated microalgal library to identify and sort variants that exhibit the desired traits such as higher growth rate and increased lipid content. By encapsulating single cells into water-in-oil emulsion droplets, each variant was separately cultured inside an individual droplet that functioned as an independent bioreactor. In conjunction with an on-chip fluorescent lipid staining process within droplets, microalgal growth and lipid content were characterized by measuring chlorophyll and BODIPY fluorescence intensities through an integrated optical detection system in a flow-through manner. Droplets containing cells with higher growth and lipid content were selectively retrieved and further analyzed off-chip. The growth and lipid content screening capabilities of the developed platform were successfully demonstrated by first carrying out proof-of-concept screening using known
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.