ArticleHeliyon2019
Exploring DNA quantity and quality from raw materials to botanical extracts.
Article in Heliyon, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Traditional System Versus DNA Barcoding in Identification of Bamboo Species: A Systematic Review.Molecular biotechnology · 2021Pooled it
- Dataset on the genetic diversity and phylogenetic relationships ofData in brief · 2026Article
- Development of molecular diagnostic methods to distinguish acerola species for quality assurance of food, dietary supplements and natural health products.Scientific reports · 2025Article
- Flower Species Ingredient Verification Using Orthogonal Molecular Methods.Foods (Basel, Switzerland) · 2024Article
- Nuclear Magnetic Resonance Fingerprints and Mini DNA Markers for the Authentication of Cinnamon Species Ingredients Used in Food and Natural Health Products.Plants (Basel, Switzerland) · 2024Article
- A novel biological sources consistency evaluation method reveals high level of biodiversity within wild natural medicine: A case study ofActa pharmaceutica Sinica. B · 2023Article
- Deoxyribonucleic Acid Barcoding for the Identification of Botanicals.Progress in the chemistry of organic natural products · 2023Article
- Next-generation sequencing and targeted quantitative real-time polymerase chain reaction for detection ofAnnals of translational medicine · 2022Article
- Working with the natural complexity: Selection and characterization of black cohosh root extract for use in toxicology testing.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2022Article
- Development of Hydrolysis Probe-Based qPCR Assays forFoods (Basel, Switzerland) · 2021Article
- Quantification of Actaea racemosa L. (black cohosh) from some of its potential adulterants using qPCR and dPCR methods.Scientific reports · 2021Article
- Validation of a Triplex Quantitative Polymerase Chain Reaction Assay for Detection and Quantification of Traditional Protein Sources,Frontiers in plant science · 2021Article
- Genome skimming and NMR chemical fingerprinting provide quality assurance biotechnology to validate Sarsaparilla identity and purity.Scientific reports · 2020Article
- A strategy for test article selection and phytochemical characterization of Echinacea purpurea extract for safety testing.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe aim of this study was to explore the variability in DNA quality and quantity along a gradient of industrial processing of botanical ingredients from raw materials to extracts.
methodsA data matrix was assembled for 1242 botanical ingredient samples along a gradient of industrial processing commonly used in the Natural Health Product (NHP) industry. Multivariate statistics was used to explore dependant variables for quality and quantity. The success of attaining a positive DNA test result along a gradient of industrial processing was compared among four biotechnologies: DNA barcoding, NGS, Sanger sequencing and qPCR.
resultsThere was considerable variance in DNA quality and quantity among the samples, which could be interpreted along a gradient from raw materials with greater quantities (50-120 ng/μL) of DNA and longer DNA (400-500bp) sequences to extracts, which were characterized by lower quantities (0.1-10.0 ng/μL) and short fragments (50-150bp).
conclusionsTargeted molecular diagnostic tests for species identity can be used in the NHP industry for raw and processed samples. Non-targeted tests or the use of NGS for any identity test needs considerable research and development and must be validated before it can be used in commercial operations as these methods are subject to considerable risk of false negative and positive results. Proper use of these tools can be used to ensure ingredient authenticity, and to avert adulteration, and contamination with plants that are a health concern. Lastly these tools can be used to prevent the exploitation of rare herbal species and the harvesting of native biodiversity for commercial purposes.
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