ArticleRegenerative therapy2015
Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells.
Article in Regenerative therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- Clinically adaptable size cartilage grafts generated from human embryonic stem cells enable tracheal repair in a porcine model.Stem cell research & therapy · 2026Article
- Unique Human Intestinal Tuft Cell Features Revealed by Pluripotent Stem Cell-Derived Mini-Guts.Gastro hep advances · 2026Article
- The feasibility of human embryonic stem cell (hESC)-derived cartilage for tracheoplasty.Regenerative therapy · 2025Article
- Highly efficient XIST reactivation in female hPSC by transient dual inhibition of TP53 and DNA methylation during Cas9 mediated genome editing.Stem cell research & therapy · 2025Article
- Modeling vascular dynamics at the initial stage of endochondral ossification on a microfluidic chip using a human embryonic-stem-cell-derived organoid.Regenerative therapy · 2025Article
- Use of iPSC-Derived Smooth Muscle Cells to Model Physiology and Pathology.Arteriosclerosis, thrombosis, and vascular biology · 2024Review
- Automated xeno-free chondrogenic differentiation from human embryonic stem cells: Enhancing efficiency and ensuring high-quality mass production.Regenerative therapy · 2024Article
- ZSCAN4-binding motif-TGCACAC is conserved and enriched in CA/TG microsatellites in both mouse and human genomes.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- Human intestinal organoid-derived PDGFRα + mesenchymal stroma enables proliferation and maintenance of LGR4 + epithelial stem cells.Stem cell research & therapy · 2024Article
- Article
- De-erosion of X chromosome dosage compensation by the editing ofCell reports methods · 2022Article
- Efficient derivation and banking of clinical-grade human embryonic stem cell lines in accordance with Japanese regulations.Regenerative therapy · 2022Article
- The microRNA cluster C19MC confers differentiation potential into trophoblast lineages upon human pluripotent stem cells.Nature communications · 2022Article
- Drug metabolic activity is a critical cell-intrinsic determinant for selection of hepatocytes during long-term culture.Stem cell research & therapy · 2022Article
- Puromycin-based purification of cells with high expression of the cytochrome P450 CYP3A4 gene from a patient with drug-induced liver injury (DILI).Stem cell research & therapy · 2022Article
- Laminin-511-derived recombinant fragment and Rho kinase inhibitor Y-27632 facilitate serial cultivation of keratinocytes differentiated from human embryonic stem cells.Regenerative therapy · 2021Article
- Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells.Scientific reports · 2021Article
- Endometrial regeneration with endometrial epithelium: homologous orchestration with endometrial stroma as a feeder.Stem cell research & therapy · 2021Article
- The hsa-miR-302 cluster controls ectodermal differentiation of human pluripotent stem cell via repression ofRegenerative therapy · 2020Article
- Research and Development Strategy for Future Embryonic Stem Cell-Based Therapy in Japan.JMA journal · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The potential applications of human embryonic stem cells (hESCs) in regenerative medicine and developmental research have made stem cell biology one of the most fascinating and rapidly expanding fields of biomedicine. The first clinical trial of hESCs in humans has begun, and the field of stem cell therapy has just entered a new era. Here, we report seven hESC lines (SEES-1, -2, -3, -4, -5, -6, and -7). Four of them were derived and maintained on irradiated human mesenchymal stem cells (hMSCs) grown in xenogeneic-free defined media and substrate. Xenogeneic-free hMSCs isolated from the subcutaneous tissue of extra fingers from individuals with polydactyly showed appropriate potentials as feeder layers in the pluripotency and growth of hESCs. In this report, we describe a comprehensive characterization of these newly derived SEES cell lines. In addition, we developed a scalable culture system for hESCs having high biological safety by using gamma-irradiated serum replacement and pharmaceutical-grade recombinant basic fibroblast growth factor (bFGF, also known as trafermin). This is first report describing the maintenance of hESC pluripotency using pharmaceutical-grade human recombinant bFGF (trafermin) and gamma-irradiated serum replacement. Our defined medium system provides a path to scalability in Good Manufacturing Practice (GMP) settings for the generation of clinically relevant cell types from pluripotent cells for therapeutic applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.