Evidence map›Paper›PMID 31245370›Full record

ArticleFrontiers in cell and developmental biology2019

Determination of a Robust Assay for Human Sperm Membrane Potential Analysis.

Carolina Baro Graf, Carla Ritagliati, Cintia Stival, Paula A Balestrini, Mariano G Buffone, Darío Krapf

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cell and developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. N-Formyl-L-aspartate mediates chemotaxis in spermFrontiers in cell and developmental biology · 2022
    Article
  7. Article
  8. CaChannels (Austin, Tex.) · 2020
    Review
  9. Review
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Carolina Baro GrafCONICET-UNR, Laboratoty of Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario, Rosario, Argentina.
Carla RitagliatiCONICET-UNR, Laboratoty of Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario, Rosario, Argentina.
Cintia StivalCONICET-UNR, Laboratoty of Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario, Rosario, Argentina.
Paula A BalestriniConsejo Nacional de Investigaciones Científicas y Tecnológicas, Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina.
Mariano G BuffoneConsejo Nacional de Investigaciones Científicas y Tecnológicas, Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina.
Darío KrapfCONICET-UNR, Laboratoty of Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario, Rosario, Argentina.
Experimental Medicine and Biology Institute · ARInstituto de Biología Molecular y Celular de Rosario · ARConsejo Nacional de Investigaciones Científicas y Técnicas · ARNational University of Rosario · AR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mammalian sperm must undergo a complex process called capacitation in order to fertilize the egg. During this process, hyperpolarization of the sperm plasma membrane has been mostly studied in mouse, and associated to its importance in the preparation to undergo the acrosome reaction (AR). However, despite the increasing evidence of membrane hyperpolarization in human sperm capacitation, no reliable techniques have been set up for its determination. In this report we describe human sperm membrane potential (

Indexed as

carbocyanine dyediSC3(5)human spermmembrane potentialpopulation fluorimetrysperm capacitation

Identifiers

PMID31245370
PMCPMC6579818
OpenAlexW2950633910

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.