Evidence map›Paper›PMID 31244343›Full record

ArticleSystems biology in reproductive medicine2019

Can trophectoderm RNA analysis predict human blastocyst competency?

Panagiotis Ntostis, Georgia Kokkali, David Iles, John Huntriss, Maria Tzetis, Helen Picton, Konstantinos Pantos, David Miller

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Systems biology in reproductive medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
4.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Environmental Exposures around Conception: Developmental Pathways Leading to Lifetime Disease Risk.International journal of environmental research and public health · 2021
    Review
  12. Article
  13. Review
  14. Article
  15. Molecular Signaling Regulating Endometrium-Blastocyst Crosstalk.International journal of molecular sciences · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Panagiotis Ntostisa Department of Discovery and Translational Science , LICAMM, University of Leeds , Leeds , UK.
Georgia Kokkalic Genesis Athens hospital , Reproductive medicine Unit , Athens , Greece.
David Ilesa Department of Discovery and Translational Science , LICAMM, University of Leeds , Leeds , UK.
John Huntrissa Department of Discovery and Translational Science , LICAMM, University of Leeds , Leeds , UK.
Maria Tzetisb Department of Medical Genetics , Medical School, National and Kapodistrian University of Athens , Athens , Greece.
Helen Pictona Department of Discovery and Translational Science , LICAMM, University of Leeds , Leeds , UK.
Konstantinos Pantosc Genesis Athens hospital , Reproductive medicine Unit , Athens , Greece.
David Millera Department of Discovery and Translational Science , LICAMM, University of Leeds , Leeds , UK.ORCID http://orcid.org/0000-0002-1709-108X
University of Leeds · GBNational and Kapodistrian University of Athens · GR

Funding

Medical Research Council MR/K020501/1
6 · The paper itself

Abstract

A systematic review of the literature showed that trophectoderm biopsy could assist in the selection of healthy embryos for uterine transfer without affecting implantation rates. However, previous studies attempting to establish the relationship between trophectoderm gene expression profiles and implantation competency using either microarrays or RNA sequencing strategies, were not sufficiently optimized to handle the exceptionally low RNA inputs available from biopsied material. In this pilot study, we report that differential gene expression in human trophectoderm biopsies assayed by an ultra-sensitive next generation RNA sequencing strategy could predict blastocyst implantation competence. RNA expression profiles from isolated human trophectoderm cells were analysed with established clinical pregnancy being the primary endpoint. Following RNA sequencing, a total of 47 transcripts were found to be significantly differentially expressed between the trophectoderm cells from successfully implanted (competent) versus unsuccessful (incompetent) blastocysts. Of these, 36 transcripts were significantly down-regulated in the incompetent blastocysts, including Hydroxysteroid 17-Beta Dehydrogenase 1 (

Indexed as

RNAAdultAneuploidyBiopsyBlastocystEmbryo ImplantationFemaleFertilization in VitroGene OntologyHumansMaternal AgeMetabolomicsPilot ProjectsSequence Analysis, RNATranscriptomeTrophoblastsRNAdifferential gene expressionimplantation competenceRNA sequencingTranscriptometrophectoderm

Identifiers

PMID31244343
PMCPMC6816490
OpenAlexW2955150255

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.