ArticleAsian Pacific journal of cancer prevention : APJCP2019
MicroRNA Isolation by Trizol-Based Method and Its Stability in Stored Serum and cDNA Derivatives.
Article in Asian Pacific journal of cancer prevention : APJCP, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Extracellular vesicle-associated and non-vesicle-associated microRNAs for early breast cancer detection: a systematic review.Frontiers in genetics · 2026Pooled it
- Utility of miRNA biomarkers in patients with acute myocardial infarction (UNIAMI)-results from a prospective pilot study.Frontiers in cardiovascular medicine · 2026Article
- Utility of microRNAs in Exhaled Breath Condensate as Diagnostic Biomarkers to Differentiate Pulmonary Sarcoidosis from Pulmonary Tuberculosis.Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG · 2025Article
- Review
- Selection and Validation of Reference Genes inPlants (Basel, Switzerland) · 2024Article
- Transcriptomic and metabolomic data of goat ovarian and uterine tissues during sexual maturation.Scientific data · 2024Article
- Examination and comparison of the RNA extraction methods using mouse serum.Biomedical reports · 2024Article
- Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities.Synthetic and systems biotechnology · 2023Article
- Serum Level of egr-miR-2a-3p as a Potential Diagnostic Biomarker for Cystic Echinococcosis.Acta parasitologica · 2023Article
- IL-6 Promotes Hepatocellular Carcinoma Invasion by Releasing Exosomal miR-133a-3p.Gastroenterology research and practice · 2022Article
- The Role of Circulating MicroRNAs in Patients with Early-Stage Pancreatic Adenocarcinoma.Biomedicines · 2021Review
- Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods.Diagnostics (Basel, Switzerland) · 2021Review
- Analysis of Age-Related Circular RNA Expression Profiles in Mesenchymal Stem Cells of Rat Bone Marrow.Frontiers in genetics · 2021Article
- MicroRNA-Based Fingerprinting of Cervical Lesions and Cancer.Journal of clinical medicine · 2020Review
- Optimal reference genes for gene expression analysis in polyploid of Cyprinus carpio and Carassius auratus.BMC genetics · 2020Article
- Clinical characteristics of 225 patients with COVID-19 in a tertiary Hospital near Wuhan, China.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- Epigenetic Programming Through Breast Milk and Its Impact on Milk-Siblings Mating.Frontiers in genetics · 2020Article
- Serum miRNAs associated with tumor-promoting cytokines in non-small cell lung cancer.PloS one · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. Since aberrant expression of miRNAs has been proposed as usage for blood-based biomarkers, hence reliable techniques for miRNA isolation as well as stability of miRNAs in various stored conditions needs to be explored. This present study aimed to investigate the efficacy of the Trizol-based isolation technique and the stability of miRNAs in stored serum and cDNA derivatives. Total RNA, including miRNAs, was isolated from human serum and a comparison of the efficiency of the Trizol®LS reagent isolation method against the miRNeasy®mini kit was conducted. Expression of RNU6, miR-145, and miR-20a was determined by quantitative real-time polymerase chain reaction (qRT-PCR). We showed that Trizol®LS isolation yielded significantly lower RNA concentrations than that of the miRNeasy®mini kit by approximately 35%. Purity of the isolated RNAs by both methods was similar. RNU6, miR-145, and miR-20a degraded at room temperature, but all genes were stable at 4ºC, -20ºC and -80ºC for a 72-hrs period, in both serum and cDNA storage conditions. In the stored cDNA derivatives, we observed the stability of RNU6, miR-145, and miR-20a for 3 months at -20ºC, and all genes also resisted 4 repeated freeze-thaw cycles at -20ºC. In conclusion, the Trizol-based method is efficient as well as economical to use for quantification of circulating miRNAs. In addition, we proposed that the storage of miRNA-derived cDNAs may be an alternative choice to avoid the stability effect.
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