Evidence map›Paper›PMID 31244227›Full record

ArticleNano letters2019

Liquid-Cell Electron Tomography of Biological Systems.

William J Dearnaley, Beatrice Schleupner, A Cameron Varano, Nick A Alden, Floricel Gonzalez, Michael A Casasanta, Birgit E Scharf, Madeline J Dukes, Deborah F Kelly

Abstract read
In one paragraph

Article in Nano letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Intermediate states of molecular self-assembly from liquid-cell electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

William J DearnaleyDepartment of Biomedical Engineering , Pennsylvania State University , University Park , Pennsylvania 16802 , United States.
Beatrice SchleupnerVirginia Tech Carilion Research Institute , Virginia Tech , Roanoke , Virginia 24016 , United States.
A Cameron VaranoDepartment of Biomedical Engineering , Pennsylvania State University , University Park , Pennsylvania 16802 , United States.
Nick A AldenDepartment of Biomedical Engineering , Pennsylvania State University , University Park , Pennsylvania 16802 , United States.
Floricel GonzalezDepartment of Biological Sciences , Virginia Tech , Blacksburg , Virginia 24061 , United States.
Michael A CasasantaDepartment of Biomedical Engineering , Pennsylvania State University , University Park , Pennsylvania 16802 , United States.
Birgit E ScharfDepartment of Biological Sciences , Virginia Tech , Blacksburg , Virginia 24061 , United States.
Madeline J DukesApplications Science , Protochips Inc. , Morrisville , North Carolina 27560 , United States.
Deborah F KellyDepartment of Biomedical Engineering , Pennsylvania State University , University Park , Pennsylvania 16802 , United States.ORCID 0000-0002-7341-7435

Funding

Multi-scale imaging of breast cancer proteins during DNA repairR01CA219700 · NCI · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI KELLY, DEBORAH F · 2018 to 2022
$2.1M
Hot Spot Analysis of the Breast Cancer Susceptibility ProteinR01CA227261 · NCI · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI KELLY, DEBORAH F · 2018 to 2022
$2.0M
Tunable Microchip Sorting of BRCA1 Nuclear AssembliesR01CA193578 · NCI · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI KELLY, DEBORAH F · 2015 to 2020
$1.8M
NCI NIH HHS R01 CA193578NCI NIH HHS R01 CA219700NCI NIH HHS R01 CA227261
6 · The paper itself

Abstract

Liquid-cell electron microscopy is a rapidly growing field in the imaging domain. While real-time observations are readily available to analyze materials and biological systems, these measurementshave been limited to the two-dimensional (2-D) image plane. Here, we introduce an exciting technical advance to image materials in 3-D while enclosed in liquid. The development of liquid-cell electron tomography permitted us to observe and quantify host-pathogen interactions in solution while contained in the vacuum system of the electron microscope. In doing so, we demonstrate new insights for the rules of engagement involving a unique bacteriophage and its host bacterium. A deeper analysis of the genetic content of the phage pathogens revealed structural features of the infectious units while introducing a new paradigm for host interactions. Overall, we demonstrate a technological opportunity to elevate research efforts for

Indexed as

AgrobacteriumBacteriophagesElectron Microscope TomographyEquipment DesignImaging, Three-DimensionalSilicon CompoundsSilicon Compoundssilicon nitridebacteriophageelectron tomographyLiquid-cellsilicon nitride

Identifiers

PMID31244227
PMCPMC6786937

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.