ArticleBMC plant biology2019
Proteomic changes in the xylem sap of Brassica napus under cadmium stress and functional validation.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 56 citations in OpenAlex.
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- Comprehensive review of multiomics applications and remediation of plant heavy metal toxicity.Stress biology · 2025Review
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- Multiomics and biotechnologies for understanding and influencing cadmium accumulation and stress response in plants.Plant biotechnology journal · 2024Review
- The Uptake, Transfer, and Detoxification of Cadmium in Plants and Its Exogenous Effects.Cells · 2024Review
- Strong Invasive Mechanism ofPlants (Basel, Switzerland) · 2024Article
- The Influence of Cadmium on Fountain Grass Performance Correlates Closely with Metabolite Profiles.Plants (Basel, Switzerland) · 2023Article
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- Mapping and Identifying Candidate Genes Enabling Cadmium Accumulation inInternational journal of molecular sciences · 2023Article
- A Green Approach Used for Heavy Metals 'Phytoremediation' Via Invasive Plant Species to Mitigate Environmental Pollution: A Review.Plants (Basel, Switzerland) · 2023Review
- Collection of Xylem Exudates from the Model PlantBio-protocol · 2022Article
- Proteomic Changes inPlants (Basel, Switzerland) · 2022Article
- Characterization of Oligopeptides inLife (Basel, Switzerland) · 2022Article
- Understanding the Phytoremediation Mechanisms of Potentially Toxic Elements: A Proteomic Overview of Recent Advances.Frontiers in plant science · 2022Review
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Proteome Changes Reveal the Protective Roles of Exogenous Citric Acid in Alleviating Cu Toxicity inInternational journal of molecular sciences · 2021Article
- Unraveling the Roles of Vascular Proteins Using Proteomics.Molecules (Basel, Switzerland) · 2021Review
- Advances in "Omics" Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants.Frontiers in plant science · 2021Review
- Genome-scale identification ofPeerJ · 2021Article
- Overexpression of a Defensin-Like GeneFrontiers in plant science · 2020Article
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2 authors at 1 institution in 1 country.
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Abstract
backgroundThe xylem sap of vascular plants primarily transports water and mineral nutrients from the roots to the shoots and also transports heavy metals such as cadmium (Cd). Proteomic changes in xylem sap is an important mechanism for detoxifying Cd by plants. However, it is unclear how proteins in xylem sap respond to Cd. Here, we investigated the effects of Cd stress on the xylem sap proteome of Brassica napus using a label-free shotgun proteomic approach to elucidate plant response mechanisms to Cd toxicity.
resultsWe identified and quantified 672 proteins; 67% were predicted to be secretory, and 11% (73 proteins) were unique to Cd-treated samples. Cd stress caused statistically significant and biologically relevant abundance changes in 28 xylem sap proteins. Among these proteins, the metabolic pathways that were most affected were related to cell wall modifications, stress/oxidoreductases, and lipid and protein metabolism. We functionally validated a plant defensin-like protein, BnPDFL, which belongs to the stress/oxidoreductase category, that was unique to the Cd-treated samples and played a positive role in Cd tolerance. Subcellular localization analysis revealed that BnPDFL is cell wall-localized. In vitro Cd-binding assays revealed that BnPDFL has Cd-chelating activity. BnPDFL heterologous overexpression significantly enhanced Cd tolerance in E. coli and Arabidopsis. Functional disruption of Arabidopsis plant defensin genes AtPDF2.3 and AtPDF2.2, which are mainly expressed in root vascular bundles, significantly decreased Cd tolerance.
conclusionsSeveral xylem sap proteins in Brassica napus are differentially induced in response to Cd treatment, and plant defensin plays a positive role in Cd tolerance.
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