ArticleEuropean heart journal2020
Transcriptional dynamics of pluripotent stem cell-derived endothelial cell differentiation revealed by single-cell RNA sequencing.
Article in European heart journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 67 citations in OpenAlex.
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- Directed Differentiation of Human Induced Pluripotent Stem Cells to Heart Valve Cells.Circulation · 2024Article
- Extracellular vesicles from differentiated stem cells contain novel proangiogenic miRNAs and induce angiogenic responses at low doses.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Transcription factors regulating vasculogenesis and angiogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Review
- Single-cell characterization of neovascularization using hiPSC-derived endothelial cells in a 3D microenvironment.Stem cell reports · 2023Article
- Single-Cell RNA-seq Analysis of a Human Embryonic Stem Cell to Endothelial Cell System Based on Transcription Factor Overexpression.Stem cell reviews and reports · 2023Article
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- Single-cell sequencing reveals homogeneity and heterogeneity of the cytopathological mechanisms in different etiology-induced AKI.Cell death & disease · 2023Article
- Epigenetic landscape reveals MECOM as an endothelial lineage regulator.Nature communications · 2023Article
- Human cortical spheroids with a high diversity of innately developing brain cell types.Stem cell research & therapy · 2023Article
- ETV2 Upregulation Marks the Specification of Early Cardiomyocytes and Endothelial Cells During Co-differentiation.Stem cells (Dayton, Ohio) · 2023Article
- Mapping the developing human cardiac endothelium at single-cell resolution identifies MECOM as a regulator of arteriovenous gene expression.Cardiovascular research · 2022Article
- A new resource for human coronary vessel development.Cardiovascular research · 2022Article
- Single-cell RNA sequencing profiling of mouse endothelial cells in response to pulmonary arterial hypertension.Cardiovascular research · 2022Article
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11 authors at 4 institutions in 1 country.
Funding
Abstract
aimsPluripotent stem cell-derived endothelial cell products possess therapeutic potential in ischaemic vascular disease. However, the factors that drive endothelial differentiation from pluripotency and cellular specification are largely unknown. The aims of this study were to use single-cell RNA sequencing (scRNA-seq) to map the transcriptional landscape and cellular dynamics of directed differentiation of human embryonic stem cell-derived endothelial cells (hESC-EC) and to compare these cells to mature endothelial cells from diverse vascular beds. METHODS AND
resultsA highly efficient directed 8-day differentiation protocol was used to generate a hESC-derived endothelial cell product (hESC-ECP), in which 66% of cells co-expressed CD31 and CD144. We observed largely homogeneous hESC and mesodermal populations at Days 0 and 4, respectively, followed by a rapid emergence of distinct endothelial and mesenchymal populations. Pseudotime trajectory identified transcriptional signatures of endothelial commitment and maturation during the differentiation process. Concordance in transcriptional signatures was verified by scRNA-seq analysis using both a second hESC line RC11, and an alternative hESC-EC differentiation protocol. In total, 105 727 cells were subjected to scRNA-seq analysis. Global transcriptional comparison revealed a transcriptional architecture of hESC-EC that differs from freshly isolated and cultured human endothelial cells and from organ-specific endothelial cells.
conclusionA transcriptional bifurcation into endothelial and mesenchymal lineages was identified, as well as novel transcriptional signatures underpinning commitment and maturation. The transcriptional architecture of hESC-ECP was distinct from mature and foetal human EC.
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