Evidence map›Paper›PMID 31242427›Full record

ArticleCell reports2019

Physical and Molecular Landscapes of Mouse Glioma Extracellular Vesicles Define Heterogeneity.

Aron Gyuris, Jose Navarrete-Perea, Ala Jo, Simona Cristea, Shuang Zhou, Kyle Fraser, Zhiyun Wei, Anna M Krichevsky, Ralph Weissleder, Hakho Lee and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 64 citations in OpenAlex.

  1. Metagenomic Data Preprocessing and Quality Control.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  2. New insights into extracellular vesicles in metastatic cancer: From mechanisms to diagnostics and targeted therapies.Molecular therapy : the journal of the American Society of Gene Therapy · 2025
    Review
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  16. Proteomics of Extracellular Vesicle in Glioblastoma.Brain tumor research and treatment · 2022
    Review
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  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Aron GyurisDepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Jose Navarrete-PereaDepartment of Cell Biology, Harvard Medical School, Boston, MA 02215, USA.
Ala JoCenter for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Simona CristeaDepartment of Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Biostatistics, Harvard T. H. Chan School of Public Health, Boston, MA 02115, USA.
Shuang ZhouDepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Kyle FraserCenter for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Zhiyun WeiDepartment of Neurology, Ann Romney Center for Neurologic Diseases, Initiative for RNA Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Anna M KrichevskyDepartment of Neurology, Ann Romney Center for Neurologic Diseases, Initiative for RNA Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Ralph WeisslederCenter for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA; Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Hakho LeeCenter for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Steve P GygiDepartment of Cell Biology, Harvard Medical School, Boston, MA 02215, USA.
Al CharestDepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA; Cancer Research Institute, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA. Electronic address: acharest@bidmc.harvard.edu.
Harvard University · USCenter for Systems Biology · USBeth Israel Deaconess Medical Center · USBrigham and Women's Hospital · USDana-Farber Cancer Institute · US

Funding

The role of microvesicles in giloma virotherapyP01CA069246 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI CHIOCCA, E. ANTONIO · 1996 to 2021
$30.9M
New Sample Multiplexing Technologies to Identify Chemical Probes and Illuminate Ubiquitin BiologyR01GM067945 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI GYGI, STEVEN P · 2003 to 2024
$10.5M
Transfer of microRNA regulators from glioblastoma to brain microenvironment U19CA179563 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI BREAKEFIELD, XANDRA OWENS, CHAREST, ALAIN · 2013 to 2017
$8.6M
(PQB5)Molecular Wiring and Therapeutic Targeting of EGFR and PDGFR Signaling NetwR01CA185137 · NCI · TUFTS MEDICAL CENTER · PI CHAREST, ALAIN, GYGI, STEVEN P · 2014 to 2017
$3.5M
NCI NIH HHS P01 CA069246NCI NIH HHS R01 CA185137NCI NIH HHS U19 CA179563NIGMS NIH HHS R01 GM067945
6 · The paper itself

Abstract

Cancer extracellular vesicles (EVs) are highly heterogeneous, which impedes our understanding of their function as intercellular communication agents and biomarkers. To deconstruct this heterogeneity, we analyzed extracellular RNAs (exRNAs) and extracellular proteins (exPTNs) from size fractionation of large, medium, and small EVs and ribonucleoprotein complexes (RNPs) from mouse glioblastoma cells by RNA sequencing and quantitative proteomics. mRNA from medium-sized EVs most closely reflects the cellular transcriptome, whereas small EV exRNA is enriched in small non-coding RNAs and RNPs contain precisely processed tRNA fragments. The exPTN composition of EVs and RNPs reveals that they are closely related by vesicle type, independent of their cellular origin, and single EV analysis reveals that small EVs are less heterogeneous in their protein content than larger ones. We provide a foundation for better understanding of segregation of macromolecules in glioma EVs through a catalog of diverse exRNAs and exPTNs.

Indexed as

AnimalsCell Line, TumorExtracellular VesiclesGlioblastomaMiceNeoplasm ProteinsRNA, NeoplasmNeoplasm ProteinsRNA, Neoplasmexosomesextracellular RNAextracellular vesiclesglioblastomamouse modelproteome

Identifiers

PMID31242427
PMCPMC6604862
OpenAlexW2954947329

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.