ArticleScientific reports2019
Purification and characterization of a novel, highly potent fibrinolytic enzyme from Bacillus subtilis DC27 screened from Douchi, a traditional Chinese fermented soybean food.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 90 citations in OpenAlex.
- Harnessing Bacillus velezensis for enhanced protease biosynthesis: optimization, purification, and kinetic profiling.BMC microbiology · 2026Article
- Proteolytic Profiles ofCurrent pharmaceutical biotechnology · 2026Article
- Research Progress on Douchi Fibrinolytic Enzyme.The protein journal · 2025Review
- Nutrition, Flavor, and Microbial Communities of Two Traditional Bacterial Douchi from Gansu, China.Foods (Basel, Switzerland) · 2024Article
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- Genetic identification and expression optimization of a novel protease HapR fromFrontiers in bioengineering and biotechnology · 2024Article
- Application of microbial enzymes in medicine and industry: current status and future perspectives.Future microbiology · 2024Review
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- Research progress on the fibrinolytic enzymes produced from traditional fermented foods.Food science & nutrition · 2023Review
- Characterization of high-yield Bacillus subtilis cysteine protease for diverse industrial applications.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Identification of Thermostable Xylose Reductase fromACS omega · 2022Article
- Chicken Feather Waste Valorization Into Nutritive Protein Hydrolysate: Role of Novel Thermostable Keratinase FromFrontiers in microbiology · 2022Article
- Metagenomic-Metabolomic Mining ofFrontiers in microbiology · 2022Article
- Urokinase-loaded cyclic RGD-decorated liposome targeted therapy forFrontiers in bioengineering and biotechnology · 2022Article
- Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications.Pharmaceutics · 2021Review
- Action of Extracellular Proteases ofLife (Basel, Switzerland) · 2021Article
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- Development and Testing of Thrombolytics in Stroke.Journal of stroke · 2021Review
- Role of Fibrinolytic Enzymes in Anti-Thrombosis Therapy.Frontiers in molecular biosciences · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The highly fibrinolytic enzyme-producing bacterium was identified as Bacillus subtilis DC27 and isolated from Douchi, a traditional fermented soybean food. The DFE27 enzyme was purified from the fermentation broth of B. subtilis DC27 by using UNOsphere Q column chromatography, Sephadex G-75 gel filtration, and high-performance liquid chromatography. It was 29 kDa in molecular mass and showed the optimal reaction temperature and pH value of 45 °C and 7.0, respectively, with a stable fibrinolytic activity below 50 °C and within the pH range of 6.0 to 10.0. DFE27 was identified as a serine protease due to its complete inhibition by phenylmethysulfony fluoride. The first 24 amino acid residues of the N-terminal sequence of the enzyme were AQSVPYGVSQIKAPALHSQGFTGS. The enzyme displayed the highest specificity toward the substrate D-Val-Leu-Lys-pNA for plasmin and it could not only directly degrade but also hydrolyze fibrin by activating plasminogen into plasmin. Overall, the DFE27 enzyme was obviously different from other known fibrinolytic enzymes in the optimum substrate specificity or fibrinolytic action mode, suggesting that it is a novel fibrinolytic enzyme and may have potential applications in the treatment and prevention of thrombosis.
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