ArticleScientific reports2019
The CYPome of the model xenobiotic-biotransforming fungus Cunninghamella elegans.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 41 citations in OpenAlex.
- From fungal diversity to antimicrobial innovation: the potential of biotransformation in the era of resistance.Frontiers in microbiology · 2026Review
- Enhanced biodegradation of fluorinated pharmaceutical by Aspergillus flavus and Cunninghamella elegans biofilms: kinetics and mechanisms.Biodegradation · 2025Article
- Genome-wide identification of CYP450 in Ganoderma lucidum and expression analysis of genes related to ganoderic acid synthesis.Archives of microbiology · 2025Article
- Rediscovering the chemistry of theRSC advances · 2024Review
- The Study of a Novel Paeoniflorin-Converting Enzyme fromMolecules (Basel, Switzerland) · 2023Article
- Comprehensive Metabolic Profiling of Euphorbiasteroid in Rats by Integrating UPLC-Q/TOF-MS and NMR as Well as Microbial Biotransformation.Metabolites · 2022Article
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- Recent Advances in Biocatalysis for Drug Synthesis.Biomedicines · 2022Review
- Robust Profiling of Cytochrome P450s (P450ome) in NotableLife (Basel, Switzerland) · 2022Article
- Nitroreduction of flutamide byBiochemistry and biophysics reports · 2022Article
- Regulation ofBiofilm · 2021Article
- Systematic characterization of metabolic profiles of ingenol in rats by UPLC-Q/TOF-MS and NMR in combination with microbial biotransformation.RSC advances · 2021Article
- Cunninghamella spp. produce mammalian-equivalent metabolites from fluorinated pyrethroid pesticides.AMB Express · 2021Article
- The Callus ofMolecules (Basel, Switzerland) · 2020Article
- Evaluation of the chemical constituents and potential biological activities ofSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fungus Cunninghamella elegans is recognised as a microbial model of mammalian drug metabolism owing to its ability to catabolise xenobiotic compounds in an analogous fashion to animals. Its ability to produce phase I (oxidative) metabolites of drugs is associated with cytochrome P450 (CYP) activity; however, almost nothing is known about these enzymes in the fungus. In this paper we report the in silico analysis of the genome sequence of C. elegans B9769, which contains 32 genes putatively coding for CYPs. Based on their predicted amino acid sequences these were classified as belonging to CYP509, 5203, 5208, 5313, 5210, 61 and 51 families. Reverse transcription-quantitative PCR revealed that the gene coding for CYP5313D1 was significantly upregulated when C. elegans DSM1908 was cultivated in sabouraud dextrose in contrast to its expression in cells grown in Roswell Park Memorial Institute medium. This corresponded to the fungus' xenobiotic biotransformation ability when grown in the two media. Heterologous expression of cyp5313D1 in Pichia pastoris resulted in a recombinant strain that biotransformed flurbiprofen to 4'-hydroxyflurbiprofen, the same metabolite generated by C. elegans cultures. This is the first report of a xenobiotic-biotransforming CYP from this biotechnologically important fungus.
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