Evidence map›Paper›PMID 31239382›Full record

ArticlemBio2019

Identification of Extracellular DNA-Binding Proteins in the Biofilm Matrix.

Jeffrey S Kavanaugh, Caralyn E Flack, Jessica Lister, Erica B Ricker, Carolyn B Ibberson, Christian Jenul, Derek E Moormeier, Elizabeth A Delmain, Kenneth W Bayles, Alexander R Horswill

Open access · goldAbstract read
In one paragraph

Article in mBio, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.

0numbers the graph read from it
0cells of the map it votes in
84citing papers in PubMed
6.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

84 citing papers in PubMed, 190 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Prevention and Treatment ofPathogens (Basel, Switzerland) · 2026
    Review
  5. Article
  6. Review
  7. Article
  8. Inflammatory Phenotypes of Bronchiectasis.Journal of personalized medicine · 2025
    Review
  9. Article
  10. Review
  11. Article
  12. Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  13. Article
  14. Review
  15. Biofilm formation byProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  16. Matrix porosity is associated withbioRxiv : the preprint server for biology · 2024
    Article
  17. Extracellular DNA-protein interactions.Current opinion in structural biology · 2024
    Review
  18. Review
  19. Article
  20. Review

24 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Jeffrey S KavanaughDepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
Caralyn E FlackDepartment of Microbiology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA.
Jessica ListerDepartment of Microbiology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA.
Erica B RickerDepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
Carolyn B IbbersonDepartment of Microbiology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA.
Christian JenulDepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
Derek E MoormeierDepartment of Microbiology and Pathology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Elizabeth A DelmainDepartment of Microbiology and Pathology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Kenneth W BaylesDepartment of Microbiology and Pathology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Alexander R HorswillDepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA alexander.horswill@ucdenver.edu.
University of Colorado Denver · USUniversity of Nebraska Medical Center · USUniversity of Iowa · USUniversity of Utah · US

Funding

The S. aureus biofilm lifecycleP01AI083211 · NIAID · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI KIELIAN, TAMMY L · 2009 to 2023
$32.1M
Vector Core-Core 2P30DK054759 · NIDDK · UNIVERSITY OF IOWA · PI Amy Leanne Ryan · 1998 to 2026
$30.5M
The molecular control of bacterial programmed cell deathR01AI125589 · NIAID · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI BAYLES, KENNETH W. · 2016 to 2020
$1.9M
Quorum Sensing Dependent Interactions with Biofilms and Innate Immunity DefensesI01BX002711 · VA · VA EASTERN COLORADO HEALTH CARE SYSTEM · PI ALEXANDER R HORSWILL · 2015 to 2026
–
BLRD VA I01 BX002711NIAID NIH HHS P01 AI083211NIAID NIH HHS R01 AI125589NIDDK NIH HHS P30 DK054759
6 · The paper itself

Abstract

We developed a new approach that couples Southwestern blotting and mass spectrometry to discover proteins that bind extracellular DNA (eDNA) in bacterial biofilms. Using

Indexed as

BiofilmsBacterial ProteinsBlotting, SouthwesternDNA, BacterialDNA-Binding ProteinsExtracellular Polymeric Substance MatrixLipoproteinsMass SpectrometryStaphylococcus aureusStatic ElectricityBacterial ProteinsDNA, BacterialDNA-Binding ProteinsLipoproteinsbiofilmseDNAextracellular DNAMRSAnucleaseSouthwestern blottingStaphylococcus aureus

Identifiers

PMID31239382
PMCPMC6593408
OpenAlexW2955483051

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.