Evidence map›Paper›PMID 31238822›Full record

ArticleOpen biology2019

MARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassembly.

Madeleine Hart, Ihsan Zulkipli, Roshan Lal Shrestha, David Dang, Duccio Conti, Parveen Gul, Izabela Kujawiak, Viji M Draviam

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Open biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Madeleine Hart1 School of Biological and Chemical Sciences, Queen Mary University of London , London , UK.
Ihsan Zulkipli2 Department of Genetics, University of Cambridge , Cambridge , UK.
Roshan Lal Shrestha2 Department of Genetics, University of Cambridge , Cambridge , UK.
David Dang1 School of Biological and Chemical Sciences, Queen Mary University of London , London , UK.
Duccio Conti1 School of Biological and Chemical Sciences, Queen Mary University of London , London , UK.
Parveen Gul1 School of Biological and Chemical Sciences, Queen Mary University of London , London , UK.
Izabela Kujawiak2 Department of Genetics, University of Cambridge , Cambridge , UK.
Viji M Draviam1 School of Biological and Chemical Sciences, Queen Mary University of London , London , UK.
Queen Mary University of London · GBUniversity of Cambridge · GB

Funding

Biotechnology and Biological Sciences Research Council BB/R01003X/1Cancer Research UK 9787Cancer Research UK C28598/A9787
6 · The paper itself

Abstract

Tissue maintenance and development requires a directed plane of cell division. While it is clear that the division plane can be determined by retraction fibres that guide spindle movements, the precise molecular components of retraction fibres that control spindle movements remain unclear. We report MARK2/Par1b kinase as a novel component of actin-rich retraction fibres. A kinase-dead mutant of MARK2 reveals MARK2's ability to monitor subcellular actin status during interphase. During mitosis, MARK2's localization at actin-rich retraction fibres, but not the rest of the cortical membrane or centrosome, is dependent on its activity, highlighting a specialized spatial regulation of MARK2. By subtly perturbing the actin cytoskeleton, we reveal MARK2's role in correcting mitotic spindle off-centring induced by actin disassembly. We propose that MARK2 provides a molecular framework to integrate cortical signals and cytoskeletal changes in mitosis and interphase.

Indexed as

ActinsCentrosomeHeLa CellsHumansMitosisMutationProtein Serine-Threonine KinasesSpindle ApparatusActinsMARK2 protein, humanProtein Serine-Threonine Kinasesactinmicrotubulesspindle orientation

Identifiers

PMID31238822
PMCPMC6597755
OpenAlexW2955316572

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.