ArticleEnvironmental microbiology2019
Genetic manipulation allows in vivo tracking of the life cycle of the son-killer symbiont, Arsenophonus nasoniae, and reveals patterns of host invasion, tropism and pathology.
Article in Environmental microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- Managing Microbiota Activity ofMicroorganisms · 2024Article
- The Genome ofInsects · 2024Article
- Insecticide susceptibility in a planthopper pest increases following inoculation with cultured Arsenophonus.The ISME journal · 2024Article
- Comparison of distinct gut bacterial communities in different stage of prediapause and nondiapause larvae inFrontiers in microbiology · 2024Article
- Article
- Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac37b) in Inner Mongolia, China.Parasite (Paris, France) · 2023Article
- Isolation, culture and characterization ofFrontiers in microbiology · 2023Article
- Abundance and Localization of Symbiotic Bacterial Communities in the Fly Parasitoid Spalangia cameroni.Applied and environmental microbiology · 2022Article
- Research Progress of the Gut Microbiome in Hybrid Fish.Microorganisms · 2022Review
- A viral mutualist employs posthatch transmission for vertical and horizontal spread among parasitoid wasps.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Sex determination systems as the interface between male-killing bacteria and their hosts.Proceedings. Biological sciences · 2022Review
- Diversity and function of arthropod endosymbiont toxins.Trends in microbiology · 2022Review
- Genetic innovations in animal-microbe symbioses.Nature reviews. Genetics · 2022Review
- Microbiome diversity and reproductive incompatibility induced by the prevalent endosymbiontEcology and evolution · 2021Article
- Transitions in symbiosis: evidence for environmental acquisition and social transmission within a clade of heritable symbionts.The ISME journal · 2021Article
- The microbiome impacts host hybridization and speciation.PLoS biology · 2021Article
- Vertical Transmission at the Pathogen-Symbiont Interface: Serratia symbiotica and Aphids.mBio · 2021Article
- When your host shuts down: larval diapause impacts host-microbiome interactions in Nasonia vitripennis.Microbiome · 2021Article
- Horizontal Transmission of Microbial Symbionts Within a Guild of Fly Parasitoids.Microbial ecology · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Maternally heritable symbionts are common in arthropods and represent important partners and antagonists. A major impediment to understanding the mechanistic basis of these symbioses has been lack of genetic manipulation tools, for instance, those enabling transgenic GFP expression systems for in vivo visualization. Here, we transform the 'son-killer' reproductive parasite Arsenophonus nasoniae that infects the parasitic wasp Nasonia vitripennis with the plasmid pOM1-gfp, re-introduce this strain to N. vitripennis and then used this system to track symbiont life history in vivo. These data revealed transfer of the symbiont into the fly pupa by N. vitripennis during oviposition and N. vitripennis larvae developing infection over time through feeding. A strong tropism of A. nasoniae to the N. vitripennis ovipositor developed during wasp pupation, which aids onward transmission. The symbiont was also visualized in diapause larvae. Occasional necrotic diapause larvae were observed which displayed intense systemic infection alongside widespread melanotic nodules indicative of an active but failed immune response. Our results provide the foundation for the study of this symbiosis through in vivo tracking of the fate of symbionts through host development, which is rarely achieved in heritable microbe/insect interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.