ArticleScientific reports2019
In vivo measurements reveal a single 5'-intron is sufficient to increase protein expression level in Caenorhabditis elegans.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Maternal histone methyltransferases antagonistically regulate autosomal random monoallelic expression (aRMAE) in C. elegans.Nature communications · 2025Article
- Reduced Cas9 transgene silencing by incorporation of intron sequences.Nature communications · 2025Article
- Introns increase gene expression in Caenorhabditis elegans by a mechanism that must be at least partly different than in plants.Scientific reports · 2025Article
- Pervasive Conservation of Intron Number and Other Genetic Elements Revealed by a Chromosome-level Genome Assembly of the Hyper-polymorphic Nematode Caenorhabditis brenneri.Genome biology and evolution · 2025Article
- Article
- Comparative analysis of new mScarlet-based red fluorescent tags in Caenorhabditis elegans.Genetics · 2024Article
- Pervasive conservation of intron number and other genetic elements revealed by a chromosome-level genomic assembly of the hyper-polymorphic nematodebioRxiv : the preprint server for biology · 2024Article
- A spatiotemporally resolved atlas of mRNA decay in thebioRxiv : the preprint server for biology · 2024Article
- Local and dynamic regulation of neuronal glycolysis in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Visualization of the biphasic calcium wave during fertilization in Caenorhabditis elegans using a genetically encoded calcium indicator.Biology open · 2023Article
- Local and dynamic regulation of neuronal glycolysisbioRxiv : the preprint server for biology · 2023Article
- O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility.PLoS genetics · 2022Article
- Intronization Signatures in Coding Exons Reveal the Evolutionary Fluidity of Eukaryotic Gene Architecture.Microorganisms · 2022Article
- Whole Exome Sequencing Reveals Genetic Variants in HLA Class II Genes Associated With Transplant-free Survival of Indeterminate Acute Liver Failure.Clinical and translational gastroenterology · 2022Article
- Article
- Calumenin knockdown, by intronic artificial microRNA, to improve expression efficiency of the recombinant human coagulation factor IX.Biotechnology letters · 2022Article
- The position of the longest intron is related to biological functions in some human genes.Frontiers in genetics · 2022Article
- Imaging of Actin Cytoskeletal Integrity During Aging in C. elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Introns control stochastic allele expression bias.Nature communications · 2021Article
- Generating Transgenics and Knockouts in Strongyloides Species by Microinjection.Journal of visualized experiments : JoVE · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Introns can increase gene expression levels using a variety of mechanisms collectively referred to as Intron Mediated Enhancement (IME). IME has been measured in cell culture and plant models by quantifying expression of intronless and intron-bearing reporter genes in vitro. We developed hardware and software to implement microfluidic chip-based gene expression quantification in vivo. We altered position, number and sequence of introns in reporter genes controlled by the hsp-90 promoter. Consistent with plant and mammalian studies, we determined a single, natural or synthetic, 5'-intron is sufficient for the full IME effect conferred by three synthetic introns, while a 3'-intron is not. We found coding sequence can affect IME; the same three synthetic introns that increase mcherry protein concentration by approximately 50%, increase mEGFP by 80%. We determined IME effect size is not greatly affected by the stronger vit-2 promoter. Our microfluidic imaging approach should facilitate screens for factors affecting IME and other intron-dependent processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.