ArticleScientific reports2019
Dynamic adaptation of mesenchymal stem cell physiology upon exposure to surface micropatterns.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Hierarchical micro-nano topographies to control Bacteria and mesenchymal stem cells biological responses.Scientific reports · 2025Article
- Bioactive Glass Microscaffolds Fabricated by Two-Photon Lithography.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Nanofibrous Membranes Based on Collagen and Conductive Polymers with Perspective for Biological Applications.Membranes · 2025Article
- Tailoring Cell Behavior and Antibacterial Properties on Zirconia Biomaterials through Femtosecond Laser-Induced Micropatterns and Nanotopography.ACS applied materials & interfaces · 2025Article
- Stem cell mechanoadaptation. I. Effect of microtubule stabilization and volume changing stresses on cytoskeletal remodeling.APL bioengineering · 2025Article
- Stem cell mechanoadaptation. II. Microtubule stabilization and substrate compliance effects on cytoskeletal remodeling.APL bioengineering · 2025Article
- Engineering Microgel Packing to Tailor the Physical and Biological Properties of Gelatin Methacryloyl Granular Hydrogel Scaffolds.Advanced healthcare materials · 2024Article
- Nanomechanical mapping of PLA hydroxyapatite composite scaffolds links surface homogeneity to stem cell differentiation.Scientific reports · 2024Article
- Article
- Microfluidically Aligned Collagen to Maintain the Phenotype of Tenocytes In Vitro.Advanced healthcare materials · 2024Article
- Advances in Regulating Cellular Behavior Using Micropatterns.The Yale journal of biology and medicine · 2023Review
- Substrate topographies modulate the secretory activity of human bone marrow mesenchymal stem cells.Stem cell research & therapy · 2023Article
- Biomechanical, biophysical and biochemical modulators of cytoskeletal remodelling and emergent stem cell lineage commitment.Communications biology · 2023Review
- Measuring cell deformation by microfluidics.Frontiers in bioengineering and biotechnology · 2023Review
- Micro-Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Heterogeneity of In Vitro Expanded Mesenchymal Stromal Cells and Strategies to Improve Their Therapeutic Actions.Pharmaceutics · 2022Review
- Biobased Elastomer Nanofibers Guide Light-Controlled Human-iPSC-Derived Skeletal Myofibers.Advanced materials (Deerfield Beach, Fla.) · 2022Article
- Cells Dynamically Adapt to Surface Geometry by Remodeling Their Focal Adhesions and Actin Cytoskeleton.Frontiers in cell and developmental biology · 2022Article
- Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer.Polymers · 2021Article
- Laser Surface Modification of Powder Metallurgy-Processed Ti-Graphite Composite Which Can Enhance Cells' Osteo-Differentiation.Materials (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human mesenchymal stem (hMSCs) are defined as multi-potent colony-forming cells expressing a specific subset of plasma membrane markers when grown on flat tissue culture polystyrene. However, as soon as hMSCs are used for transplantation, they are exposed to a 3D environment, which can strongly impact cell physiology and influence proliferation, differentiation and metabolism. Strategies to control in vivo hMSC behavior, for instance in stem cell transplantation or cancer treatment, are skewed by the un-physiological flatness of the standard well plates. Even though it is common knowledge that cells behave differently in vitro compared to in vivo, only little is known about the underlying adaptation processes. Here, we used micrometer-scale defined surface topographies as a model to describe the phenotype of hMSCs during this adaptation to their new environment. We used well established techniques to compare hMSCs cultured on flat and topographically enhanced polystyreneand observed dramatically changed cell morphologies accompanied by shrinkage of cytoplasm and nucleus, a decreased overall cellular metabolism, and slower cell cycle progression resulting in a lower proliferation rate in cells exposed to surface topographies. We hypothesized that this reduction in proliferation rate effects their sensitivity to certain cancer drugs, which was confirmed by higher survival rate of hMSCs cultured on topographies exposed to paclitaxel. Thus, micro-topographies can be used as a model system to mimic the natural cell micro-environment, and be a powerful tool to optimize cell treatment in vitro.
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