ArticleVirology journal2019
Comparing the yield of oropharyngeal swabs and sputum for detection of 11 common pathogens in hospitalized children with lower respiratory tract infection.
Article in Virology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- Pediatric Respiratory Syncytial Virus Diagnostic Testing Performance: A Systematic Review and Meta-analysis.The Journal of infectious diseases · 2023Pooled it
- Immunochromatography for the diagnosis of Mycoplasma pneumoniae infection: A systematic review and meta-analysis.PloS one · 2020Pooled it
- Enhanced Detection of Community-Acquired Pneumonia Pathogens With the BioFire® Pneumonia FilmArray® Panel.Diagnostic microbiology and infectious disease · 2021Trial
- The prevalence and role of human respiratory syncytial virus in pediatric respiratory tract infections: a systematic review and meta-analysis of global data.EClinicalMedicine · 2026Article
- Respiratory Infection-Related Pathogens in the Pediatric Intensive Care Unit During 2019-2024 in Hubei, China.Pathogens (Basel, Switzerland) · 2026Article
- Shifts in the Lung Microbiota and Antibiotic Resistance Genes Occur With Aging in Patients With Lower Respiratory Tract Infections.BioMed research international · 2026Article
- Risk factors for re-hospitalization within 90 days of discharge for severe influenza in children.BMC infectious diseases · 2025Article
- Targeted next-generation sequencing for pediatric lower respiratory tract infections: a retrospective study.Frontiers in cellular and infection microbiology · 2025Article
- A comparative study of general and severe mycoplasma pneumoniae pneumonia in children.BMC infectious diseases · 2024Article
- Respiratory syncytial virus subtypes in children with bronchiolitis: does it correlate with clinical severity?BMC infectious diseases · 2024Article
- Epidemiology and clinical severity of the serotypes of human parainfluenza virus in children with acute respiratory infection.Virology journal · 2023Article
- Article
- Comparative study of diagnostic efficacy of sputum and bronchoalveolar lavage fluid specimens in community-acquired pneumonia children treated with fiberoptic bronchoscopy.BMC infectious diseases · 2023Article
- Epidemiology of respiratory syncytial virus in hospitalized children with community-acquired pneumonia in Guangzhou: a 10-year study.Journal of thoracic disease · 2023Article
- High incidence of the virus among respiratory pathogens in children with lower respiratory tract infection in northwestern China.Journal of medical virology · 2023Article
- A Comparative Study of Severe and Critical Influenza B in Children in the 2021-2022 Winter Season.International journal of general medicine · 2022Article
- Comparison of microbiota in the upper versus lower respiratory tract in children during health and respiratory disease: protocol for a systematic review.Systematic reviews · 2021Article
- Article
- Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP-based multiplex-PCR assay.Journal of clinical laboratory analysis · 2020Article
- Point-of-Care Testing by Multiplex-PCR in Different Compartments in Suspected Lower Respiratory Tract Infection After Lung Transplantation-Results of a Prospective Study.Transplant infectious disease : an official journal of the Transplantation SocietyArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAdvances in molecular laboratory techniques are changing the prospects for the diagnosis of viral infectious diseases. Multiplex polymerase chain reaction assay (multiplex-PCR) can detect dozens of pathogens simultaneously, greatly reducing turnaround time (TAT) and improving detection sensitivity. But as a double-edged sword, due to the high sensitivity of PCR, the type of respiratory specimens is critical to diagnosis. In this work, we performed a head-to-head comparison to evaluate the multiplex-PCR yields between two samples, sputum and flocked oropharyngeal swabs (OPS).
methodsEleven common respiratory pathogens were tested in hospitalized children< 13 years of age who met the criteria for lower respiratory tract infection by GeXP-based multiplex-PCR of paired OPS and sputum.
resultsFrom January to June 2018, 440 children with paired OPS and sputum were tested. The positive rate was 84% (369/440) for OPS and 88% (386/440) for sputum (p = .007). The frequency of detection of HRV, RSV, Influenza A virus, HMPV, parainfluenza virus, adenovirus, M. pneumoniae, coronavirus, bocavirus and C. pneumoniae in sputa was higher than that of OPSs (all p < .001). Both types of specimens had similarly very good kappa values for most of pathogens, except for Mycoplasma pneumonia (κ = 0.61) and Chlamydia pneumoniae (κ = 0.24). Additionally, 79.3% (349/440) of cases showed consistent results between the two types of samples, and they were significantly younger than patients with inconsistent results (p = .002).
conclusionsFlocked oropharyngeal swabs and sputum performed similarly for the detection of common respiratory pathogens in hospitalized children by multiplex-PCR, except for Mycoplasma pneumoniae and Chlamydia pneumoniae. Young patients are likely to have consistent results between the two specimens.
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