ArticleMalaria journal2019
Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors.
Article in Malaria journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 15 citations in OpenAlex.
- Low-Abundance and FragmentaryBiomolecules · 2026Article
- Field evaluation of a loop-mediated isothermal amplification test for detection ofCurrent research in parasitology & vector-borne diseases · 2026Article
- Assessing the threat of artemisinin resistance: seasonal and ecological patterns ofFrontiers in cellular and infection microbiology · 2026Article
- Leveraging genomic insights from the neglected malaria parasites P. malariae and P. ovale using selective whole genome amplification (SWGA) approach.BMC genomics · 2025Article
- Epidemiology and likelihood of asymptomatic malaria among community dwellers in the Fanteakwa south district of Ghana.Parasite epidemiology and control · 2024Article
- Comparative evaluation of the diagnostic accuracies of four different malaria rapid diagnostic test kits available in Ghana.PloS one · 2024Article
- Molecular speciation of Plasmodium and multiplicity of P. falciparum infection in the Central region of Ghana.PLOS global public health · 2024Article
- The use of the WHO criteria to detect severe malaria among patients clinically diagnosed with uncomplicated malaria.PLOS global public health · 2024Article
- Utilization of 18s ribosomal RNA LAMP for detecting Plasmodium falciparum in microscopy and rapid diagnostic test negative patients.PloS one · 2022Article
- Effect of AsymptomaticIndian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2021Article
- Article
- Characterization of putative drug resistant biomarkers in Plasmodium falciparum isolated from Ghanaian blood donors.BMC infectious diseases · 2020Article
- Elevated IL-12, TNF-Journal of immunology research · 2020Article
- Profiling antimalarial drug-resistant haplotypes inTherapeutic advances in infectious diseaseArticle
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5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundUndesirable consequences of donor Plasmodium falciparum parasitaemia on stored donor blood have been reported. Therefore, it is imperative that all prospective blood donors are screened for P. falciparum infections using sensitive techniques. In this study, the sensitivities of microscopy, rapid diagnostic test (RDT), loop-mediated isothermal amplification (LAMP) assay and selective whole genome amplification (sWGA) technique in detecting P. falciparum infections in blood donors was assessed.
methodsRandomly selected blood donors from 5 districts in Greater Accra Region of Ghana were screened for asymptomatic P. falciparum infections. Each donor sample was screened with SD Bioline RDT kit for P. falciparum histidine rich protein 2 and Plasmodium lactate dehydrogenase antigens, sWGA and 18s-rRNA LAMP. Crude DNA LAMP (crDNA-LAMP) was compared to purified DNA LAMP (pDNA-LAMP).
resultsA total of 771 blood donors were screened. The respective overall prevalence of P. falciparum in Ghana by microscopy, RDT, crDNA-LAMP, pDNA-LAMP and sWGA was 7.4%, 11.8%, 16.9%, 17.5% and 18.0%. Using sWGA as the reference test, the sensitivities of microscopy, RDT, crDNA-LAMP and pDNA-LAMP were 41.0% (95% CI 32.7-49.7), 65.5% (95% CI 56.9-73.3), 82.6% (95% CI 75.8-88.3) and 95.7% (95% CI 90.1-98.4), respectively. There was near perfect agreement between LAMP and sWGA (sWGA vs. crDNA-LAMP, κ = 0.87; sWGA vs. pDNA-LAMP, κ = 0.96), while crDNA-LAMP and pDNA-LAMP agreed perfectly (κ = 0.91). Goodness of fit test indicated non-significant difference between the performance of LAMP and sWGA (crDNA-LAMP vs. sWGA: x
conclusionsLAMP assay agreed near perfectly with sWGA with non-significant differences in their ability to detect asymptomatic P. falciparum parasitaemia in blood donors. Therefore, it is recommended that LAMP based assays are employed to detect P. falciparum infections in blood donors due to its high sensitivity, simplicity, cost-effectiveness and user-friendliness.
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