ArticleJournal of visualized experiments : JoVE2019
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution.
Article in Journal of visualized experiments : JoVE, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Experience-Dependent Gain Modulation Drives Thermosensory Responses in Behavior.bioRxiv : the preprint server for biology · 2026Article
- ImmatureProceedings of the National Academy of Sciences of the United States of America · 2026Article
- ImmaturebioRxiv : the preprint server for biology · 2025Article
- Exploring neurodevelopment via spatiotemporal collation of anatomical networks with NeuroSC.eLife · 2025Article
- NeuroSC: Exploring Neurodevelopment via Spatiotemporal Collation of Anatomical Networks.bioRxiv : the preprint server for biology · 2025Article
- Article
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- Article
- A light sheet fluorescence microscopy protocol forFrontiers in cell and developmental biology · 2022Article
- Delineating the mechanisms and design principles ofComputational and structural biotechnology journal · 2022Review
- Multiview confocal super-resolution microscopy.Nature · 2021Article
- REMBI: Recommended Metadata for Biological Images-enabling reuse of microscopy data in biology.Nature methods · 2021Article
- Article
- Multi-tissue patterning drives anterior morphogenesis of the C. elegans embryo.Developmental biology · 2021Article
- Rapid image deconvolution and multiview fusion for optical microscopy.Nature biotechnology · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
Caenorhabditis elegans (C. elegans) stands out as the only organism in which the challenge of understanding the cellular origins of an entire nervous system can be observed, with single cell resolution, in vivo. Here, we present an integrated protocol for the examination of neurodevelopment in C. elegans embryos. Our protocol combines imaging, lineaging and neuroanatomical tracing of single cells in developing embryos. We achieve long-term, four-dimensional (4D) imaging of living C. elegans embryos with nearly isotropic spatial resolution through the use of Dual-view Inverted Selective Plane Illumination Microscopy (diSPIM). Nuclei and neuronal structures in the nematode embryos are imaged and isotropically fused to yield images with resolution of ~330 nm in all three dimensions. These minute-by-minute high-resolution 4D data sets are then analyzed to correlate definitive cell-lineage identities with gene expression and morphological dynamics at single-cell and subcellular levels of detail. Our protocol is structured to enable modular implementation of each of the described steps and enhance studies on embryogenesis, gene expression, or neurodevelopment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.