ArticleFrontiers in nutrition2019
An Improved Enzyme-Linked Immunosorbent Assay (ELISA) Based Protocol Using Seeds for Detection of Five Major Peanut Allergens Ara h 1, Ara h 2, Ara h 3, Ara h 6, and Ara h 8.
Article in Frontiers in nutrition, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 32 citations in OpenAlex.
- Genetic regulation of major immunogenic protein accumulation in peanut seeds.Functional & integrative genomics · 2026Article
- Food Allergen Carryover Within the Edible Insect Supply Chain: A Pilot Plant Investigation.Foods (Basel, Switzerland) · 2026Article
- Food Allergenicity Evaluation Methods: Classification, Principle, and Applications.Foods (Basel, Switzerland) · 2025Review
- Peanut Genotypes with Reduced Content of Immunogenic Proteins by Breeding, Biotechnology, and Management: Prospects and Challenges.Plants (Basel, Switzerland) · 2025Article
- A novel IgE epitope-specific antibodies-based sandwich ELISA for sensitive measurement of immunoreactivity changes of peanut allergen Ara h 2 in processed foods.Frontiers in nutrition · 2024Article
- Detection of Peanut Traces in Food by an Official Food Safety Laboratory.Foods (Basel, Switzerland) · 2022Article
- An Ultrasensitive Silicon-Based Electrolyte-Gated Transistor for the Detection of Peanut Allergens.Biosensors · 2022Article
- Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices.Foods (Basel, Switzerland) · 2021Review
- Arachis hypogaea gene expression atlas for fastigiata subspecies of cultivated groundnut to accelerate functional and translational genomics applications.Plant biotechnology journal · 2020Article
- Translational genomics for achieving higher genetic gains in groundnut.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Use of IHF-QD Microscopic Analysis for the Detection of Food Allergenic Components: Peanuts and Wheat Protein.Foods (Basel, Switzerland) · 2020Article
- Advances in Crop Improvement and Delivery Research for Nutritional Quality and Health Benefits of Groundnut (Frontiers in plant science · 2020Review
- Hypoallergen Peanut Lines Identified Through Large-Scale Phenotyping of Global Diversity Panel: Providing Hope Toward Addressing One of the Major Global Food Safety Concerns.Frontiers in genetics · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peanut allergy is an important health concern among many individuals. As there is no effective treatment to peanut allergy, continuous monitoring of peanut-based products, and their sources is essential. Precise detection of peanut allergens is key for identification and development of improved peanut varieties with minimum or no allergens in addition to estimating the levels in peanut-based products available in food chain. The antibody based ELISA protocol along with sample preparation was standardized for Ara h 1, Ara h 2, Ara h 3, Ara h 6, and Ara h 8 to estimate their quantities in peanut seeds. Three different dilutions were optimized to precisely quantify target allergen proteins in peanut seeds such as Ara h 1 (1/1,000, 1/2,000, and 1/4,000), Ara h 2 and Ara h 3 (1/5,000, 1/10,000, and 1/20,000), Ara h 6 (1/40,000, 1/80,000, and 1/1,60,000), and Ara h 8 (1/10, 1/20, and 1/40). These dilutions were finalized for each allergen based on the accuracy of detection by achieving <20% coefficient of variation in three technical replicates. This protocol captured wide variation of allergen proteins in selected peanut genotypes for Ara h 1 (77-46,106 μg/g), Ara h 2 (265-5,426 μg/g), Ara h 3 (382-12,676 μg/g), Ara h 6 (949-43,375 μg/g), and Ara h 8 (0.385-6 μg/g). The assay is sensitive and reliable in precise detection of five major peanut allergens in seeds. Deployment of such protocol allows screening of large scale germplasm and breeding lines while developing peanut varieties with minimum allergenicity to ensure food safety.
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