ArticleFrontiers in genetics2019
Next Generation Sequencing for Long Non-coding RNAs Profile for CD4
Article in Frontiers in genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Review
- Functional sequence variants of intergenic long noncoding RNA on chromosome 17q21 are associated with asthma.The European respiratory journal · 2025Article
- Non-Coding RNAs in Asthma: Regulators of Eosinophil Biology and Airway Inflammation.Diagnostics (Basel, Switzerland) · 2025Review
- LincR-PPP2R5C Deficiency Alleviates Airway Remodeling by Inhibiting Epithelial-Mesenchymal Transition Through the PP2A/TGF-β1 Signaling Pathway in Chronic Experimental Allergic Asthma.Allergy, asthma & immunology research · 2024Article
- LincR-PPP2R5C Promotes Th2 Cell Differentiation Through PPP2R5C/PP2A by Forming an RNA-DNA Triplex in Allergic Asthma.Allergy, asthma & immunology research · 2024Article
- Studies on the role of non-coding RNAs in controlling the activity of T cells in asthma.Non-coding RNA research · 2023Review
- Review
- Progresses in epigenetic studies of asthma from the perspective of high-throughput analysis technologies: a narrative review.Annals of translational medicine · 2022Review
- Evolving Views of Long Noncoding RNAs and Epigenomic Control of Lymphocyte State and Memory.Cold Spring Harbor perspectives in biology · 2022Review
- Emerging Roles of Non-Coding RNAs in Childhood Asthma.Frontiers in pharmacology · 2022Review
- A systematic evaluation of bioinformatics tools for identification of long noncoding RNAs.RNA (New York, N.Y.) · 2021Article
- Genome-wide association study reveals a novel locus for asthma with severe exacerbations in diverse populations.Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2021Article
- LINC01198 facilitates gliomagenesis through activating PI3K/AKT pathway.RNA biology · 2020Article
- Long Noncoding RNAs in the Regulation of Asthma: Current Research and Clinical Implications.Frontiers in pharmacology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsAlthough long non-coding RNAs (lncRNAs) have been linked to many diseases including asthma, little is known about lncRNA transcriptomes of CD4
methodsNext generation sequencing for lncRNAs and mRNAs was performed on CD4+ T cells from asthma and control mice. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway analyses were performed to predict the functions and signal pathways for the aberrant lncRNAs. The selected lncRNAs were further measured using quantitative real-time PCR (polymerase chain reaction) and observed in the fluorescence
resultsNext generation sequencing revealed 36 up-regulated lncRNAs and 98 down-regulated lncRNAs in acute asthma compared with controls. KEGG pathway analysis showed that cytokine-cytokine receptor interaction had the highest enrichment scores. A co-expression network was constructed in which 23 lncRNAs and 301 mRNAs altered formed a total of 12424 lncRNA and mRNA pairs. To validate the RNA sequencing results, we measured the 4 different lncRNAs using qPCR. The lncRNA fantom3_9230106C11 was significantly reduced in CD4+ T cells of asthma. Bioinformatics analysis showed that lncRNA fantom3_9230106C11 had the potential to interact with many miRNAs and transcription factors related to Th2 differentiation.
conclusionThis study provided the first evidence for different expression of lncRNAs of CD4+T cells in asthma and may serve as a template for further, larger functional in-depth analyses regarding asthma molecular lncRNAs.
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