ArticleCell host & microbe2019
A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability.
Article in Cell host & microbe, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
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Who cites it
114 citing papers in PubMed.
- Mysterious girls: The biology of female Cryptosporidium parasites.PLoS pathogens · 2026Article
- Lessons on multicellular two- (2D) and three-dimensional (3D) culture in parasitology: Insights, challenges and future directions.International journal for parasitology · 2026Review
- Long-term feeder cell-free cat intestinal organoid cultures to study Toxoplasma gondii's sexual development.Communications biology · 2026Article
- Insights into the lifecycle of Cryptosporidium and compounds targeting developmental stages.Current opinion in microbiology · 2026Review
- In Vitro Culture of Cryptosporidium spp. Using Stem Cell-Derived Intestinal Epithelial Monolayers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Defining enteric bacterial pathogenesis using organoids:Gut microbes · 2025Article
- Pathogenicity and virulence ofVirulence · 2025Review
- Structural and functional characterization of TgGSK3, a druggable kinase in Toxoplasma gondii.Nature communications · 2025Article
- Independent evolution of holocentric chromosomes in an early branching apicomplexan parasite.bioRxiv : the preprint server for biology · 2025Article
- Fibroblasts enhance the growth and survival of adult feline small intestinal organoids.mSphere · 2025Article
- Cryptosporidium parvum multidrug resistance protein confers resistance to toxic gut microbial metabolite.Cell host & microbe · 2025Article
- Cryptosporidium parvum protease INS6 plays an important role in parasite proliferation and pathogenicity.PLoS neglected tropical diseases · 2025Article
- Novel organoids andParasitology · 2025Review
- Organoid-basedMicrobiology spectrum · 2025Article
- Innovations in intestinal organoid technology featuring an open apical surface.European journal of cell biology · 2025Review
- SKSR1 identified as key virulence factor in Cryptosporidium by genetic crossing.Nature communications · 2025Article
- Review
- SAMD9 senses cytosolic double-stranded nucleic acids in epithelial and mesenchymal cells to induce antiviral immunity.Nature communications · 2025Article
- Leveraging microphysiological systems to expedite understanding of host-parasite interactions.PLoS pathogens · 2025Review
54 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors.
Funding
Abstract
Despite being a frequent cause of severe diarrheal disease in infants and an opportunistic infection in immunocompromised patients, Cryptosporidium research has lagged due to a lack of facile experimental methods. Here, we describe a platform for complete life cycle development and long-term growth of C. parvum in vitro using "air-liquid interface" (ALI) cultures derived from intestinal epithelial stem cells. Transcriptomic profiling revealed that differentiating epithelial cells grown under ALI conditions undergo profound changes in metabolism and development that enable completion of the parasite life cycle in vitro. ALI cultures support parasite expansion > 100-fold and generate viable oocysts that are transmissible in vitro and to mice, causing infection and animal death. Transgenic parasite lines created using CRISPR/Cas9 were used to complete a genetic cross in vitro, demonstrating Mendelian segregation of chromosomes during meiosis. ALI culture provides an accessible model that will enable innovative studies into Cryptosporidium biology and host interactions.
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