ArticleJournal of industrial microbiology & biotechnology2019
Efficient production of levan using a recombinant yeast Saccharomyces cerevisiae hypersecreting a bacterial levansucrase.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Recent Advances in Microbial Levan Production: Strategies for Yield Enhancement and Sustainability.International journal of food science · 2026Review
- Ultrasonic-assisted enzymatic extraction, physicochemical properties and prebiotic activities of polysaccharides from Saccharomyces cerevisiae spore wall.Ultrasonics sonochemistry · 2025Article
- Insight of Metal Ions in Enzymatic Synthesis of Levan: The Metal-Binding Loop.Journal of microbiology and biotechnology · 2025Article
- Expanding the horizons of levan: from microbial biosynthesis to applications and advanced detection methods.World journal of microbiology & biotechnology · 2024Review
- Levan Production byMolecules (Basel, Switzerland) · 2024Article
- Engineering strategies for enhanced heterologous protein production by Saccharomyces cerevisiae.Microbial cell factories · 2024Review
- Recent Developments and Applications of Microbial Levan, A Versatile Polysaccharide-Based Biopolymer.Molecules (Basel, Switzerland) · 2023Review
- Exopolysaccharides Producing Bacteria: A Review.Microorganisms · 2023Review
- Fructan Biosynthesis by Yeast Cell Factories.Journal of microbiology and biotechnology · 2022Review
- A novel protein fusion partner, carbohydrate-binding module family 66, to enhance heterologous protein expression in Escherichia coli.Microbial cell factories · 2021Article
- Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae.Biotechnology for biofuels · 2021Article
- Yeast Synthetic Biology for the Production ofMolecules (Basel, Switzerland) · 2021Review
- Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Levan is a fructose polymer with diverse applications in the food and medical industries. In this study, levansucrase from Rahnella aquatilis (RaLsrA) was hyper-secreted using a Saccharomyces cerevisiae protein secretion system. An optimal secretion signal, a translation fusion partner (TFP) containing an N-terminal 98 amino acid domain from a mitochondrial inner membrane protein, UTH1, was employed to secrete approximately 50 U/mL of bioactive RaLsrA into culture media with 63% secretion efficiency by fed-batch fermentation. Although the purified RaLsrA was useful for enzymatic conversion of high-molecular-weight levan of approximately 3.75 × 10
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