Evidence map›Paper›PMID 31228550›Full record

ArticleMethods (San Diego, Calif.)2020

Biochemical characterization of RNA-guided ribonuclease activities for CRISPR-Cas9 systems.

Max J Gramelspacher, Zhonggang Hou, Yan Zhang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods (San Diego, Calif.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Max J GramelspacherDepartment of Biological Chemistry, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI 48109, USA.
Zhonggang HouDepartment of Biological Chemistry, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI 48109, USA.
Yan ZhangDepartment of Biological Chemistry, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI 48109, USA. Electronic address: yzhangbc@med.umich.edu.
University of Michigan–Ann Arbor · US

Funding

The alternative roles of Neisseria CRISPR-Cas9 in endogenous gene regulation and bacterial physiologyR00GM117268 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ZHANG, YAN · 2017 to 2019
$747k
The alternative roles of Neisseria CRISPR-Cas9 in endogenous gene regulation and bacterial physiologyK99GM117268 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI ZHANG, YAN · 2016 to 2017
$104k
NIGMS NIH HHS K99 GM117268NIGMS NIH HHS R00 GM117268
6 · The paper itself

Abstract

The majority of bacteria and archaea rely on CRISPR-Cas systems for RNA-guided, adaptive immunity against mobile genetic elements. The Cas9 family of type II CRISPR-associated DNA endonucleases generates programmable double strand breaks in the CRISPR-complementary DNA targets flanked by the PAM motif. Nowadays, CRISPR-Cas9 provides a set of powerful tools for precise genome manipulation in eukaryotes and prokaryotes. Recently, a few Cas9 orthologs have been reported to possess intrinsic CRISPR-guided, sequence-specific ribonuclease activities. These discoveries fundamentally expanded the targeting capability of CRISPR-Cas9 systems, and promise to provide new CRISPR tools to manipulate specific cellular RNA transcripts. Here we present a detailed method for the biochemical characterization of Cas9's RNA-targeting potential.

Indexed as

BacteriophagesCRISPR-Associated Protein 9CRISPR-Cas SystemsEnzyme AssaysNeisseria meningitidisRNA, Guide, CRISPR-Cas SystemsViral ProteinsCRISPR-Associated Protein 9RNA, Guide, CRISPR-Cas SystemsViral Proteins

Identifiers

PMID31228550
PMCPMC6923617
OpenAlexW2953319031

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.