ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2020
Amplifying Bone Marrow Progenitors Expressing α-Smooth Muscle Actin Produce Zonal Insertion Sites During Tendon-to-Bone Repair.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Optimizing strategies in tendon tissue engineering through effective scaffold design: overview of recent advancements.Regenerative medicine · 2026Review
- Localized Delivery of a Small Molecule Hedgehog Agonist via Poly(ε-caprolactone) Scaffolds Enhances Tendon-to-Bone Integration.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Recent advances in gradient biomimetic scaffolds for tendon-bone interface regeneration.Frontiers in bioengineering and biotechnology · 2025Review
- Sustained release of ubiquitin-like protein ISG-15 enhances tendon-to-bone healing following anterior cruciate ligament reconstruction in a mouse model.Frontiers in bioengineering and biotechnology · 2025Article
- Repair potential of self-assembling peptide hydrogel in a mouse model of anterior cruciate ligament reconstruction.Journal of experimental orthopaedics · 2024Article
- Synovium-Derived Mesenchymal Stem Cell-Based Scaffold-Free Fibrocartilage Engineering for Bone-Tendon Interface Healing in an Anterior Cruciate Ligament Reconstruction Model.Tissue engineering and regenerative medicine · 2024Article
- Preclinical tendon and ligament models: Beyond the 3Rs (replacement, reduction, and refinement) to 5W1H (why, who, what, where, when, how).Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Review
- Targeting the hedgehog signaling pathway to improve tendon-to-bone integration.Osteoarthritis and cartilage · 2023Article
- Scaffold-based tissue engineering strategies for soft-hard interface regeneration.Regenerative biomaterials · 2023Review
- Gravity-based patterning of osteogenic factors to preserve bone structure after osteochondral injury in a large animal model.Biofabrication · 2022Article
- Article
- Multiplexed tape-stabilized cryohistology of mineralized large animal specimens.Journal of biological methods · 2022Article
- Effects of tamoxifen on tendon homeostasis and healing: Considerations for the use of tamoxifen-inducible mouse models.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Tmem100- and Acta2-Lineage Cells Contribute to Implant Osseointegration in a Mouse Model.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2021Article
- Cells from a GDF5 origin produce zonal tendon-to-bone attachments following anterior cruciate ligament reconstruction.Annals of the New York Academy of Sciences · 2020Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Traditional tendon-to-bone repair where the tendon is reattached to bone via suture anchors often results in disorganized scar production rather than the formation of a zonal insertion. In contrast, ligament reconstructions where tendon grafts are passed through bone tunnels can yield zonal tendon-to-bone attachments between the graft and adjacent bone. Therefore, ligament reconstructions can be used to study mechanisms that regulate zonal tendon-to-bone repair in the adult. Anterior cruciate ligament (ACL) reconstructions are one of the most common reconstruction procedures and while we know that cells from outside the graft produce the attachments, we have not yet established specific cell populations that give rise to this tissue. To address this knowledge gap, we performed ACL reconstructions in lineage tracing mice where α-smooth muscle actin (αSMACreERT2) was used to label αSMA-expressing progenitors within the bone marrow that produced zonal attachments. Expression of αSMA was increased during early stages of the repair process such that the contribution of SMA-labeled cells to the tunnel integration was highest when tamoxifen was delivered in the first week post-surgery. The zonal attachments shared features with normal entheses, including tidemarks oriented perpendicularly to collagen fibers, Col1a1-expressing cells, alkaline phosphatase activity, and proteoglycan-rich staining. Finally, the integration strength increased with time, requiring 112% greater force to remove the graft from the tunnel at 28 days compared with 14 days post-surgery. Future studies will target these progenitor cells to define the pathways that regulate zonal tendon-to-bone repair in the adult. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:105-116, 2020.
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